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Glyoxalase I (GLO1) Human Recombinant Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845142

Background: GLO1 is involved in the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione. GLO1 is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. GLO1 enzyme is abundantly… More details

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Technical Datasheet View PDF
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Product Overview

Product Name
Glyoxalase I (GLO1), Active Protein
Full Product Name
Human Recombinant Glyoxalase I
Product Gene Name
Product Synonym Names
Lactoylglutathione lyase; Glx 1; GLO-1 Methylglyoxalase; Aldoketomutase; Human Recombinant Glyoxalase I
Sequence
MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM
Host
E Coli
Form/Format
1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 10% glycerol.
Appearance: Liquid
Purity/Purification
>=90%
Concentration
1 mg/ml (lot specific)
Preparation and Storage
At -80 degree C
Shelf Life: 12 months
Molecular Weight
20.7 kDa (184 aa, 1-184 aa)
OMIM
138750
3D Structure
ModBase 3D Structure for Q04760
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Specific activity: >0.4 units/mg.
Results
>0.4 units/mg
Unit Definition
One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25 degree C. Specific activity was expressed in units/mg of protein.
Handling
Centrifuge the vial prior to opening.
Applications Tested/Suitable for GLO1 active protein
SDS-PAGE

Testing Data of GLO1 active protein

Human Recombinant GLO1 Active Protein – Cat# MBS845142

Other Notes

Small volumes of GLO1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GLO1 purchase

MBS845142 is an active protein targeting GLO1. Also known as Human Recombinant Glyoxalase I. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GLO1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
lactoylglutathione lyase
NCBI Official Synonym Full Names
glyoxalase I
NCBI Official Symbol
GLO1
NCBI Official Synonym Symbols
GLYI; GLOD1; HEL-S-74
UniProt Protein Name
Lactoylglutathione lyase
UniProt Synonym Protein Names
Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase
UniProt Gene Name
GLO1
UniProt Synonym Gene Names
Glx I
UniProt Entry Name
LGUL_HUMAN
NCBI Protein Information
lactoylglutathione lyase

NCBI Summary for GLO1

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]

UniProt Comments for GLO1

GLO1: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Belongs to the glyoxalase I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Carbohydrate Metabolism - pyruvate; EC 4.4.1.5

Chromosomal Location of Human Ortholog: 6p21.3-p21.1

Cellular Component: cytoplasm

Molecular Function: zinc ion binding; lactoylglutathione lyase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; glutathione metabolic process; carbohydrate metabolic process; osteoclast differentiation; negative regulation of apoptosis; methylglyoxal metabolic process

Disease: Autism
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GLO1 active protein

 Reference Product  PubMed Publications
 HAGH active protein  >4 publications with GLO1 and HAGH
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