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TPO Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845170

Background: TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TPO, Active Protein
Full Product Name
TPO, rat recombinant
Product Gene Name
TPO active protein
Product Synonym Names
Thrombopoietin; Megakaryocyte colony-stimulating factor; c-MPL Ligand; MGDF
Sequence
SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF
Host
E Coli
Form/Format
Sterile filtered through a 0.2 micron filter. Lyophilized from Sodium Citrate, pH 4.5
Appearance: Lyophilized powder
Purity/Purification
>=98%
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
18.7 kDa
3D Structure
ModBase 3D Structure for P49745
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
Endotoxin Level
< 0.1 ng/mug of protein (<1EU/mug).
Activity (Specifications/test method)
Determined by its ability to stimulate the proliferation of human MO7e cells. The expected ED50 is <= 0.2 ng/ml, corresponding to a specific activity of >= 5 x 10^6 units/mg.
Biological Activity
Determined by its ability to stimulate the proliferation of human MO7e cells. The expected ED50 is <= 0.2 ng/ml, corresponding to a specific activity of >= 5 x 10^6 units/mg.
Handling
Centrifuge the vial prior to opening.

Product Categories/Family for TPO active protein

Cytokines; Growth Factors & Hormones; TPOsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; TPOsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth
Applications Tested/Suitable for TPO active protein
SDS-PAGE; HPLC

Other Notes

Small volumes of TPO active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPO purchase

MBS845170 is an active protein targeting TPO. Also known as TPO, rat recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TPO. It may not necessarily be applicable to this product.

NCBI GI #
13592113
NCBI GeneID
81811
NCBI Accession #
NP_112395.1
UniProt Primary Accession #
P49745
UniProt Related Accession #
P49745
GenBank Nucleotide #
NM_031133.2
NCBI Official Full Name
thrombopoietin
NCBI Official Synonym Full Names
thrombopoietin
NCBI Official Symbol
Thpo
UniProt Protein Name
Thrombopoietin
Protein Family
Thyroid peroxidase
UniProt Gene Name
Thpo
UniProt Entry Name
TPO_RAT
NCBI Protein Information
thrombopoietin

NCBI Summary for TPO

lineage-specific cytokine; important for proliferation and development of megakaryocytes and platelets; may be the major regulator of circulating platelets [RGD, Feb 2006]

UniProt Comments for TPO

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Secreted; Cell cycle regulation

Cellular Component: extracellular space; extracellular region

Molecular Function: hormone activity; cytokine activity; receptor binding

Biological Process: cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; myeloid cell differentiation; response to lipopolysaccharide; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation
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Research Articles on TPO

1. The data suggest a potential role of thrombopoietin (TPO) as a physiological regulator of coronary flow. By acting on specific receptors present on endothelial cells, TPO may induce PI3K/Akt-dependent eNOS phosphorylation and NO release.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TPO active protein

 Reference Product  PubMed Publications
 Mpl active protein  >30 publications with TPO and Mpl
 Jak2 active protein  >3 publications with TPO and Jak2
 Bcl2l1 active protein  >2 publications with TPO and Bcl2l1
 Stat3 active protein  >1 publications with TPO and Stat3

Diseases associated with TPO active protein

 Disease Name  Pubmed Publications
 Thrombocytosis Antibodies  >11 publications with TPO and Thrombocytosis
 Anemia Antibodies  >8 publications with TPO and Anemia
 Hemorrhage Antibodies  >3 publications with TPO and Hemorrhage
 Lung Neoplasms Antibodies  >3 publications with TPO and Lung Neoplasms
 Congenital Abnormalities Antibodies  >3 publications with TPO and Congenital Abnormalities
 Growth Disorders Antibodies  >2 publications with TPO and Growth Disorders
 Carcinoma, Squamous Cell Antibodies  >2 publications with TPO and Carcinoma, Squamous Cell
 Thrombocythemia, Essential Antibodies  >2 publications with TPO and Thrombocythemia, Essential
 Neoplasms, Experimental Antibodies  >1 publications with TPO and Neoplasms, Experimental
 Hypertension Antibodies  >1 publications with TPO and Hypertension

Organs/Tissues associated with TPO active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >39 publications with TPO and Brain
 Kidney Antibodies  >7 publications with TPO and Kidney
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