GFRA2/GDNFRB (GFRA2) Active Protein

Name: GFRA2 Active Protein; GFRA2/GDNFRB; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS845213
Price: 335 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845213

Background: GDNF family receptor alpha-2 (GFRA2) also known as GDNF receptor beta, Neurturin receptor alpha, RET ligand 2, TGF-beta-related neurotrophic factor receptor 2, is a cell membrane protein which belongs to the GDNFR family. Glial cell line-derived… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

GFRA2/GDNFRB, Active Protein

Full Product Name

Human CellExp GFRA2 / GDNFRB, human recombinant

Product Gene Name

GFRA2 active protein

Product Synonym Names

GFRA2; GDNFRB; NRTNR-ALPHA; NTNRA; RETL2; TRNR2.

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 47.6 kDa. The predicted N-terminus is Ser 22. DTT-reduced Protein migrates as 60-70 KDa due to glycosylation.

OMIM

601956

3D Structure

ModBase 3D Structure for O00451

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Neurturin at 1 ug/ml can bind rhGFRA2 with an apparent KD < 8 nM.

Results

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Neurturin at 1 ug/ml can bind rhGFRA2 with an apparent KD < 8 nM.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for GFRA2 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Other Proteins & Enzymes

Applications Tested/Suitable for GFRA2 active protein

SDS-PAGE

Other Notes

Small volumes of GFRA2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GFRA2 purchase

MBS845213 is an active protein targeting GFRA2. Also known as Human CellExp GFRA2 / GDNFRB, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for GFRA2. It may not necessarily be applicable to this product.

NCBI GI #

259089450

NCBI GeneID

2675

NCBI Accession #

NP_001158510.1

UniProt Primary Accession #

O00451

UniProt Secondary Accession #

O15316; O15328; Q58J92; Q6GTR9; Q7Z5C2; E9PD47

UniProt Related Accession #

O00451

GenBank Nucleotide #

NM_001165038.1

NCBI Official Full Name

GDNF family receptor alpha-2 isoform b

NCBI Official Synonym Full Names

GDNF family receptor alpha 2

NCBI Official Symbol

GFRA2

NCBI Official Synonym Symbols

NTNRA; RETL2; TRNR2; GDNFRB; NRTNR-ALPHA

UniProt Protein Name

GDNF family receptor alpha-2

UniProt Synonym Protein Names

GDNF receptor beta; GDNFR-beta; Neurturin receptor alpha; NRTNR-alpha; NTNR-alpha; RET ligand 2; TGF-beta-related neurotrophic factor receptor 2

Protein Family

GDNF family receptor

UniProt Gene Name

GFRA2

UniProt Synonym Gene Names

GDNFRB; RETL2; TRNR2; GDNF receptor alpha-2; GDNFR-alpha-2; GFR-alpha-2; GDNFR-beta; NRTNR-alpha; NTNR-alpha

UniProt Entry Name

GFRA2_HUMAN

NCBI Protein Information

GDNF family receptor alpha-2

NCBI Summary for GFRA2

Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

UniProt Comments for GFRA2

GFRA2: Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: extrinsic to membrane; plasma membrane

Molecular Function: glial cell line-derived neurotrophic factor receptor activity

Biological Process: nervous system development; negative regulation of protein amino acid autophosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway

Research Articles on GFRA2

1. In the cochlea, immunolabeling for GFRalpha-2 receptors was identified mainly in the cell bodies of the spiral neurons than in the nerve fibers. No structures in the organ of Corti were labeled with GFRalpha-2 receptor antibody

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GFRA2 active protein

Reference Product	PubMed Publications
GDNF active protein	>109 publications with GFRA2 and GDNF
RET active protein	>93 publications with GFRA2 and RET
NRTN active protein	>43 publications with GFRA2 and NRTN
ARTN active protein	>4 publications with GFRA2 and ARTN
PSPN active protein	>4 publications with GFRA2 and PSPN

Diseases associated with GFRA2 active protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>24 publications with GFRA2 and Nervous System Diseases
Brain Diseases Antibodies	>14 publications with GFRA2 and Brain Diseases
Disease Models, Animal Antibodies	>8 publications with GFRA2 and Disease Models, Animal
Pain Antibodies	>7 publications with GFRA2 and Pain
Cardiovascular Diseases Antibodies	>6 publications with GFRA2 and Cardiovascular Diseases
Nerve Degeneration Antibodies	>5 publications with GFRA2 and Nerve Degeneration
Movement Disorders Antibodies	>4 publications with GFRA2 and Movement Disorders
Atrophy Antibodies	>3 publications with GFRA2 and Atrophy
Mental Disorders Antibodies	>3 publications with GFRA2 and Mental Disorders
Seizures Antibodies	>2 publications with GFRA2 and Seizures

Organs/Tissues associated with GFRA2 active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>50 publications with GFRA2 and Brain
Embryonic Tissue Antibodies	>15 publications with GFRA2 and Embryonic Tissue
Eye Antibodies	>12 publications with GFRA2 and Eye
Kidney Antibodies	>11 publications with GFRA2 and Kidney
Intestine Antibodies	>8 publications with GFRA2 and Intestine
Muscle Antibodies	>8 publications with GFRA2 and Muscle
Bone Antibodies	>4 publications with GFRA2 and Bone
Testis Antibodies	>3 publications with GFRA2 and Testis
Prostate Antibodies	>2 publications with GFRA2 and Prostate
Heart Antibodies	>2 publications with GFRA2 and Heart