GFRA1/GDNFRA (GFRA1) Active Protein

Name: GFRA1 Active Protein; GFRA1/GDNFRA; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS845224
Price: 335 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845224

Background: GDNF family receptor alpha-1 (GFRA1) also known as RET ligand 1 (RETL1), TGF-beta-related neurotrophic factor receptor 1 (TRNR1), is a novel glycosylphosphatidylinositol (GPI)-linked cell surface receptor, and belongs to the glial cell… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

GFRA1/GDNFRA, Active Protein

Full Product Name

Human CellExp GFRA1 / GDNFRA, human recombinant

Product Gene Name

GFRA1 active protein

Product Synonym Names

GFRA1; GDNFRA; RETL1; TRNR1; GDNFR; GFR-ALPHA-1; RET1L

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 45.5 kDa. The predicted N-terminus is Asp 25. DTT-reduced Protein migrates as 55-60 kDa due to glycosylation.

OMIM

601496

3D Structure

ModBase 3D Structure for P56159

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GDNF at 1 ug/mL binds rhGFRA1 with an apparent KD <10 nM.

Results

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GDNF at 1 ug/mL binds rhGFRA1 with an apparent KD <10 nM.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for GFRA1 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Other Proteins & Enzymes

Applications Tested/Suitable for GFRA1 active protein

SDS-PAGE

Other Notes

Small volumes of GFRA1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GFRA1 purchase

MBS845224 is an active protein targeting GFRA1. Also known as Human CellExp GFRA1 / GDNFRA, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for GFRA1. It may not necessarily be applicable to this product.

NCBI GI #

224282178

NCBI GeneID

2674

NCBI Accession #

NP_001138925.1

UniProt Primary Accession #

P56159

UniProt Secondary Accession #

O15507; O43912; A8KA21

UniProt Related Accession #

P56159

GenBank Nucleotide #

NM_001145453.1

NCBI Official Full Name

GDNF family receptor alpha-1 isoform b preproprotein

NCBI Official Synonym Full Names

GDNF family receptor alpha 1

NCBI Official Symbol

GFRA1

NCBI Official Synonym Symbols

GDNFR; RET1L; RETL1; TRNR1; GDNFRA; GFR-ALPHA-1

UniProt Protein Name

GDNF family receptor alpha-1

UniProt Synonym Protein Names

RET ligand 1; TGF-beta-related neurotrophic factor receptor 1

Protein Family

GDNF family receptor

UniProt Gene Name

GFRA1

UniProt Synonym Gene Names

GDNFRA; RETL1; TRNR1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1

UniProt Entry Name

GFRA1_HUMAN

NCBI Protein Information

GDNF family receptor alpha-1

NCBI Summary for GFRA1

Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Feb 2009]

UniProt Comments for GFRA1

GFRA1: Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: extrinsic to membrane; plasma membrane

Molecular Function: receptor binding; glial cell line-derived neurotrophic factor receptor activity

Biological Process: axon guidance; cell surface receptor linked signal transduction

Research Articles on GFRA1

1. Methylation changes of GFRA1, SRF, and ZNF382 may be a potential biomarker set for prediction of gastric carcinoma metastasis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GFRA1 active protein

Reference Product	PubMed Publications
GDNF active protein	>318 publications with GFRA1 and GDNF
RET active protein	>240 publications with GFRA1 and RET
NRTN active protein	>36 publications with GFRA1 and NRTN
NCAM1 active protein	>17 publications with GFRA1 and NCAM1
SRC active protein	>11 publications with GFRA1 and SRC
ARTN active protein	>9 publications with GFRA1 and ARTN
PSPN active protein	>5 publications with GFRA1 and PSPN

Pathways associated with GFRA1 active protein

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Diurnally Regulated Genes With Circadian Orthologs Pathway antibodies	Diurnally Regulated Genes With Circadian Orthologs Pathway Diagram
NCAM Signaling For Neurite Out-growth Pathway antibodies	NCAM Signaling For Neurite Out-growth Pathway Diagram
NCAM1 Interactions Pathway antibodies	NCAM1 Interactions Pathway Diagram
Signaling Events Regulated By Ret Tyrosine Kinase Pathway antibodies	Signaling Events Regulated By Ret Tyrosine Kinase Pathway Diagram

Diseases associated with GFRA1 active protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>57 publications with GFRA1 and Nervous System Diseases
Disease Models, Animal Antibodies	>18 publications with GFRA1 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>16 publications with GFRA1 and Cardiovascular Diseases
Adenocarcinoma Antibodies	>14 publications with GFRA1 and Adenocarcinoma
Kidney Diseases Antibodies	>7 publications with GFRA1 and Kidney Diseases
Nerve Degeneration Antibodies	>5 publications with GFRA1 and Nerve Degeneration
Necrosis Antibodies	>5 publications with GFRA1 and Necrosis
Inflammation Antibodies	>4 publications with GFRA1 and Inflammation
Neoplasms, Experimental Antibodies	>4 publications with GFRA1 and Neoplasms, Experimental
Breast Neoplasms Antibodies	>4 publications with GFRA1 and Breast Neoplasms

Organs/Tissues associated with GFRA1 active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>99 publications with GFRA1 and Brain
Testis Antibodies	>61 publications with GFRA1 and Testis
Ganglia Antibodies	>47 publications with GFRA1 and Ganglia
Embryonic Tissue Antibodies	>31 publications with GFRA1 and Embryonic Tissue
Kidney Antibodies	>29 publications with GFRA1 and Kidney
Blood Antibodies	>17 publications with GFRA1 and Blood
Muscle Antibodies	>17 publications with GFRA1 and Muscle
Intestine Antibodies	>12 publications with GFRA1 and Intestine
Eye Antibodies	>11 publications with GFRA1 and Eye
Bone Antibodies	>7 publications with GFRA1 and Bone