VEGF-B (VEGFB) Active Protein

Name: VEGFB Active Protein; VEGF-B; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS845227
Price: 320 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845227

Background: VEGF-B, a member of the VEGF family, is a potent growth and angiogenic cytokine. It promotes DNA synthesis in endothelial cells, regulates angiogenesis and Vascular permeability, and inhibits apoptosis in certain smooth muscle cells and neurons.… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

VEGF-B (VEGFB), Active Protein

Full Product Name

VEGF-B, human recombinant

Product Gene Name

VEGFB active protein

Product Synonym Names

Vascular Endothelial Growth Factor-B; VEGFB; VEGF-related factor

Host

E Coli

Form/Format

Lyophilized without additives
Appearance: Lyophilized protein

Purity/Purification

>=98%

Preparation and Storage

Store the lyophilized VEGFB at -20 degrees C. Reconstituted VEGFB should be stored in working aliquots at -70 degrees C.

Molecular Weight

19.63 kDa

OMIM

601398

3D Structure

ModBase 3D Structure for P49765

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Reconstitute in 0.1% acetic acid to a concentration of 0.1-1.0 mg/ml. The solution should be stored at -70 degree C.

Endotoxin Level

<0.1 ng/ug

Biological Activity

Per researcher's feedback, the protein is biologically active as determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) in the presence of human VEGF165. The expected ED50 for this effect is 0.5-5.0 ug/ml.

Handling

Centrifuge the vial prior to opening.

Applications Tested/Suitable for VEGFB active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of VEGFB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VEGFB purchase

MBS845227 is an active protein targeting VEGFB. Also known as VEGF-B, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for VEGFB. It may not necessarily be applicable to this product.

NCBI GI #

344179103

NCBI GeneID

7423

NCBI Accession #

NP_001230662.1

UniProt Primary Accession #

P49765

UniProt Secondary Accession #

Q16528

UniProt Related Accession #

P49765

GenBank Nucleotide #

NM_001243733.1

NCBI Official Full Name

vascular endothelial growth factor B isoform VEGFB-167

NCBI Official Synonym Full Names

vascular endothelial growth factor B

NCBI Official Symbol

VEGFB

NCBI Official Synonym Symbols

VRF; VEGFL

UniProt Protein Name

Vascular endothelial growth factor B

UniProt Synonym Protein Names

VEGF-related factor; VRF

Protein Family

Vascular endothelial growth factor

UniProt Gene Name

VEGFB

UniProt Synonym Gene Names

VRF; VEGF-B; VRF

UniProt Entry Name

VEGFB_HUMAN

NCBI Protein Information

vascular endothelial growth factor B

NCBI Summary for VEGFB

This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011]

UniProt Comments for VEGFB

VEGFB: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell cycle regulation; Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: heparin binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; vascular endothelial growth factor receptor 1 binding; chemoattractant activity

Biological Process: response to drug; platelet activation; protein amino acid O-linked glycosylation; positive regulation of vascular permeability; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; platelet degranulation; positive chemotaxis; positive regulation of cell division; positive regulation of endothelial cell proliferation; negative regulation of neuron apoptosis; angiogenesis; vascular endothelial growth factor receptor signaling pathway; blood coagulation; negative regulation of apoptosis; cardiac muscle contraction

Research Articles on VEGFB

1. MMP9 may activate VEGF-B via PI3K/Akt signaling pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with VEGFB active protein

Reference Product	PubMed Publications
VEGFA active protein	>56 publications with VEGFB and VEGFA
VEGFC active protein	>23 publications with VEGFB and VEGFC
FLT1 active protein	>13 publications with VEGFB and FLT1
KDR active protein	>9 publications with VEGFB and KDR
PGF active protein	>5 publications with VEGFB and PGF
FLT4 active protein	>4 publications with VEGFB and FLT4
NRP1 active protein	>4 publications with VEGFB and NRP1
FIGF active protein	>2 publications with VEGFB and FIGF

Pathways associated with VEGFB active protein

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Heart Development Pathway antibodies	Heart Development Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram
Pathways In Cancer antibodies	Pathways In Cancer Diagram

Diseases associated with VEGFB active protein

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>11 publications with VEGFB and Heart Diseases
Neoplasm Invasiveness Antibodies	>7 publications with VEGFB and Neoplasm Invasiveness
Hypertrophy Antibodies	>7 publications with VEGFB and Hypertrophy
Edema Antibodies	>7 publications with VEGFB and Edema
Inflammation Antibodies	>7 publications with VEGFB and Inflammation
Pancreatic Neoplasms Antibodies	>6 publications with VEGFB and Pancreatic Neoplasms
Liver Diseases Antibodies	>5 publications with VEGFB and Liver Diseases
Breast Neoplasms Antibodies	>5 publications with VEGFB and Breast Neoplasms
Neoplasm Metastasis Antibodies	>5 publications with VEGFB and Neoplasm Metastasis
Hypertension Antibodies	>5 publications with VEGFB and Hypertension

Organs/Tissues associated with VEGFB active protein

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>98 publications with VEGFB and Vascular
Blood Antibodies	>40 publications with VEGFB and Blood
Heart Antibodies	>16 publications with VEGFB and Heart
Muscle Antibodies	>14 publications with VEGFB and Muscle
Brain Antibodies	>11 publications with VEGFB and Brain
Placenta Antibodies	>9 publications with VEGFB and Placenta
Lung Antibodies	>9 publications with VEGFB and Lung
Liver Antibodies	>7 publications with VEGFB and Liver
Eye Antibodies	>7 publications with VEGFB and Eye
Kidney Antibodies	>5 publications with VEGFB and Kidney