Human Recombinant UCHL5 Active Protein – Cat# MBS845238

UCHL5 Human Recombinant Active Protein

Name: UCHL5 Active Protein; Human Recombinant; MyBioSource
Brand: MyBioSource
SKU: MBS845238
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845238

Background: UCHL5 is a member of the peptidase C12 family. It is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and is the deubiquitinating enzyme related to the 19S regulatory subunit of the 26S proteasome. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

UCHL5, Active Protein

Full Product Name

Human Recombinant UCHL5

Product Gene Name

UCHL5 active protein

Product Synonym Names

Ubiquitin carboxyl-terminal hydrolase isozyme L5; UCH37; Human Recombinant UCHL5

Sequence

MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK

Host

E Coli

Form/Format

0.5 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 5 mM DTT, 200 mM NaCl, 0.1 mM PMSF, 2 mM EDTA and 30% glycerol.
Appearance: Liquid

Purity/Purification

>=90%

Concentration

0.5 mg/ml (lot specific)

Preparation and Storage

At -80 degree C
Shelf Life: 12 months

Molecular Weight

39.7 kDa (349 aa, 1-329 aa + NT His Tag)

OMIM

610667

3D Structure

ModBase 3D Structure for Q9Y5K5

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity: >500 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37 degree C.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for UCHL5 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

Applications Tested/Suitable for UCHL5 active protein

SDS-PAGE

Other Notes

Small volumes of UCHL5 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UCHL5 purchase

MBS845238 is an active protein targeting UCHL5. Also known as Human Recombinant UCHL5. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for UCHL5. It may not necessarily be applicable to this product.

NCBI GI #

312922359

NCBI GeneID

51377

NCBI Accession #

NP_001186190.1

UniProt Primary Accession #

Q9Y5K5

UniProt Secondary Accession #

Q5LJA6; Q5LJA7; Q8TBS4; Q96BJ9; Q9H1W5; Q9P0I3; Q9UQN2

UniProt Related Accession #

Q9Y5K5

GenBank Nucleotide #

NM_001199261.1

NCBI Official Full Name

ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 2

NCBI Official Synonym Full Names

ubiquitin carboxyl-terminal hydrolase L5

NCBI Official Symbol

UCHL5

NCBI Official Synonym Symbols

UCH37; CGI-70; INO80R; UCH-L5

UniProt Protein Name

Ubiquitin carboxyl-terminal hydrolase isozyme L5

UniProt Synonym Protein Names

Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5

Protein Family

Ubiquitin carboxyl-terminal hydrolase

UniProt Gene Name

UCHL5

UniProt Synonym Gene Names

UCH37; UCH-L5

UniProt Entry Name

UCHL5_HUMAN

NCBI Protein Information

ubiquitin carboxyl-terminal hydrolase isozyme L5

UniProt Comments for UCHL5

UCHL5: Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. Belongs to the peptidase C12 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Ubiquitin-specific protease; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: proteasome complex; cytosol; nucleus

Molecular Function: omega peptidase activity; protein binding; endopeptidase inhibitor activity; ubiquitin-specific protease activity

Biological Process: ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of transcription, DNA-dependent; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; midbrain development; DNA repair; forebrain morphogenesis; negative regulation of transforming growth factor beta receptor signaling pathway; lateral ventricle development; DNA recombination

Research Articles on UCHL5

1. Uch37 consists of two domains, a globular UCH-domain and a fibrous C-terminal tail. The C-terminal residues of Uch37 are implicated in Rpn13 binding.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with UCHL5 active protein

Reference Product	PubMed Publications
ADRM1 active protein	>14 publications with UCHL5 and ADRM1
PSMD14 active protein	>1 publications with UCHL5 and PSMD14
PSMD4 active protein	>1 publications with UCHL5 and PSMD4

Pathways associated with UCHL5 active protein

Products by Pathway	Pathway Diagram
Downregulation Of TGF-beta Receptor Signaling Pathway antibodies	Downregulation Of TGF-beta Receptor Signaling Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By TGF-beta Receptor Complex Pathway antibodies	Signaling By TGF-beta Receptor Complex Pathway Diagram
TGF-beta Receptor Signaling Activates SMADs Pathway antibodies	TGF-beta Receptor Signaling Activates SMADs Pathway Diagram

Diseases associated with UCHL5 active protein

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>3 publications with UCHL5 and Adenocarcinoma
Carcinoma, Hepatocellular Antibodies	>2 publications with UCHL5 and Carcinoma, Hepatocellular
Inflammation Antibodies	>2 publications with UCHL5 and Inflammation
Liver Neoplasms Antibodies	>2 publications with UCHL5 and Liver Neoplasms
Breast Neoplasms Antibodies	>2 publications with UCHL5 and Breast Neoplasms
Carcinoma, Squamous Cell Antibodies	>2 publications with UCHL5 and Carcinoma, Squamous Cell
Neoplasm Metastasis Antibodies	>2 publications with UCHL5 and Neoplasm Metastasis
Anemia Antibodies	>1 publications with UCHL5 and Anemia
Lung Neoplasms Antibodies	>1 publications with UCHL5 and Lung Neoplasms
Cell Transformation, Neoplastic Antibodies	>1 publications with UCHL5 and Cell Transformation, Neoplastic

Organs/Tissues associated with UCHL5 active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>3 publications with UCHL5 and Liver
Kidney Antibodies	>2 publications with UCHL5 and Kidney
Blood Antibodies	>2 publications with UCHL5 and Blood
Uterus Antibodies	>1 publications with UCHL5 and Uterus
Lung Antibodies	>1 publications with UCHL5 and Lung
Skin Antibodies	>1 publications with UCHL5 and Skin
Bone Antibodies	>1 publications with UCHL5 and Bone
Spleen Antibodies	>1 publications with UCHL5 and Spleen
Intestine Antibodies	>1 publications with UCHL5 and Intestine
Ovary Antibodies	>1 publications with UCHL5 and Ovary