• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Pf-4/CXCL4 (CXCL4) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845254

Background: PF-4 is a CXC chemokine that is expressed in megakaryocytes and stored in the alpha-granules of platelets. PF-4 is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. Recombinant murine PF-4 is an 8.2 kDa… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
PF-4/CXCL4, Active Protein
Full Product Name
PF-4 / CXCL4, murine recombinant
Product Gene Name
CXCL4 active protein
Product Synonym Names
Platelet Factor-4; CXCL4; Oncostatin A; Ironplact
Sequence
VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES
Host
E Coli
Form/Format
Sterile filtered through a 0.2 micron filter. Lyophilized with no additives
Appearance: Lyophilized powder
Purity/Purification
>=98%
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
10.3 kDa
3D Structure
ModBase 3D Structure for Q9Z126
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
Endotoxin Level
< 0.1 ng/mug of protein (<1EU/mug).
Activity (Specifications/test method)
Determined by its ability to chemoattract human neutrophils using a concentration range of 10.0-100.0 ng/ml.
Biological Activity
Determined by its ability to chemoattract human neutrophils using a concentration range of 10.0-100.0 ng/ml.
Handling
Centrifuge the vial prior to opening.

Product Categories/Family for CXCL4 active protein

Cytokines; Growth Factors & Hormones; ChemokinesCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Chemokines Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytok
Applications Tested/Suitable for CXCL4 active protein
SDS-PAGE; HPLC

Other Notes

Small volumes of CXCL4 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CXCL4 purchase

MBS845254 is an active protein targeting CXCL4. Also known as PF-4 / CXCL4, murine recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CXCL4. It may not necessarily be applicable to this product.

NCBI GI #
9910486
NCBI GeneID
56744
NCBI Accession #
NP_064316.1
UniProt Primary Accession #
Q9Z126
UniProt Related Accession #
Q9Z126
GenBank Nucleotide #
NM_019932.4
NCBI Official Full Name
platelet factor 4
NCBI Official Synonym Full Names
platelet factor 4
NCBI Official Symbol
Pf4
NCBI Official Synonym Symbols
Cxcl4; Scyb4
UniProt Protein Name
Platelet factor 4
UniProt Synonym Protein Names
C-X-C motif chemokine 4
UniProt Gene Name
Pf4
UniProt Synonym Gene Names
Cxcl4; Scyb4; PF-4
UniProt Entry Name
PLF4_MOUSE
NCBI Protein Information
platelet factor 4

UniProt Comments for CXCL4

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Apoptosis; Secreted, signal peptide

Cellular Component: extracellular space; protein complex; extracellular region; vesicle

Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity; CXCR chemokine receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; platelet activation; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; chemotaxis; positive regulation of tumor necrosis factor production; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; negative regulation of MHC class II biosynthetic process; protein complex assembly; immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; positive regulation of macrophage differentiation
Show more

Research Articles on CXCL4

1. Data indicate that platelet factor 4 (PF4) is involved directly in liver innate immune response against ischaemia-reperfusion injury (IRI) by regulating Th17 cell differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CXCL4 active protein

 Reference Product  PubMed Publications
 Vwf active protein  >39 publications with CXCL4 and Vwf
 Ppbp active protein  >17 publications with CXCL4 and Ppbp
 Cxcr3 active protein  >14 publications with CXCL4 and Cxcr3
 Proc active protein  >13 publications with CXCL4 and Proc
 Alb active protein  >3 publications with CXCL4 and Alb
 Plg active protein  >2 publications with CXCL4 and Plg
 Hrg active protein  >2 publications with CXCL4 and Hrg

Pathways associated with CXCL4 active protein

 Products by Pathway  Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Common Pathway Of Fibrin Clot Formation antibodies  Common Pathway Of Fibrin Clot Formation Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram

Diseases associated with CXCL4 active protein

 Disease Name  Pubmed Publications
 Thrombocytopenia Antibodies  >496 publications with CXCL4 and Thrombocytopenia
 Cardiovascular Diseases Antibodies  >375 publications with CXCL4 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >351 publications with CXCL4 and Vascular Diseases
 Thrombosis Antibodies  >327 publications with CXCL4 and Thrombosis
 Heart Diseases Antibodies  >120 publications with CXCL4 and Heart Diseases
 Inflammation Antibodies  >82 publications with CXCL4 and Inflammation
 Nervous System Diseases Antibodies  >68 publications with CXCL4 and Nervous System Diseases
 Autoimmune Diseases Antibodies  >62 publications with CXCL4 and Autoimmune Diseases
 Kidney Diseases Antibodies  >58 publications with CXCL4 and Kidney Diseases
 Atherosclerosis Antibodies  >56 publications with CXCL4 and Atherosclerosis

Organs/Tissues associated with CXCL4 active protein

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >109 publications with CXCL4 and Heart
 Bone Antibodies  >80 publications with CXCL4 and Bone
 Bone Marrow Antibodies  >71 publications with CXCL4 and Bone Marrow
 Liver Antibodies  >38 publications with CXCL4 and Liver
 Embryonic Tissue Antibodies  >18 publications with CXCL4 and Embryonic Tissue
 Spleen Antibodies  >17 publications with CXCL4 and Spleen
 Joint Antibodies  >8 publications with CXCL4 and Joint
 Thymus Antibodies  >4 publications with CXCL4 and Thymus
 Prostate Antibodies  >4 publications with CXCL4 and Prostate
 Uterus Antibodies  >3 publications with CXCL4 and Uterus
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000