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T4 DNA Ligase Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845261

Background: T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
T4 DNA Ligase, Recombinant Protein
Full Product Name
T4 DNA Ligase (5 u / mul)
Product Synonym Names
T4 DNA Ligase
Host
E Coli
Form/Format
ENZYME STORAGE BUFFER: 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 5 mM DTT, 50% glycerol (v/v).
Appearance: Liquid
Concentration
5 U/ul (lot specific)
Preparation and Storage
At -80 degree C
Shelf Life: 12 months
Unit Definition
One unit of T4 DNA ligase is the amount of enzyme activity required to ligate EcoR I digested 1.0 ug of plasmid DNA in 5 minutes at 20 degree C in a 20 ul Reaction.
Handling
Centrifuge the vial prior to opening.
Applications Tested/Suitable for T4 DNA Ligase recombinant protein
SDS-PAGE; HPLC

Other Notes

Small volumes of T4 DNA Ligase recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for T4 DNA Ligase purchase

MBS845261 is a recombinant protein targeting T4 DNA Ligase. Also known as T4 DNA Ligase (5 u / mul). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for T4 DNA Ligase. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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