Carbonic Anhydrase 9/CA9 (CA9) Active Protein

Name: CA9 Active Protein; Carbonic Anhydrase 9/CA9; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS845333
Price: 385 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845333

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Carbonic Anhydrase 9/CA9, Active Protein

Full Product Name

Human CellExp Carbonic Anhydrase 9 / CA9, human recombinant

Product Gene Name

CA9 active protein

Product Synonym Names

CAIX; CA9; CA-IX; G250; MN; P54/58N; pMW1

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in 20 mM MES and 100 mM NaCl, pH 6.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 41.2 kDa. The predicted N-terminus is Gln 38. DTT-reduced Protein migrates as 53-54 kDa due to glycosylation.

OMIM

603179

3D Structure

ModBase 3D Structure for Q16790

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by its esterase activity. The specific activity is measured with 1 mM 4-Nitrophenyl acetate and 2.5 mug enzyme at 400 nm in 100 muL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is > 15 pmoles / min / mug.

Results

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for CA9 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Other Proteins & EnzymesDiabetes; Obesity & Metabolic Syndrome; Lipids Metabolism; Proteins/Enzymes; Other (NEW)

Applications Tested/Suitable for CA9 active protein

SDS-PAGE

Other Notes

Small volumes of CA9 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CA9 purchase

MBS845333 is an active protein targeting CA9. Also known as Human CellExp Carbonic Anhydrase 9 / CA9, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CA9. It may not necessarily be applicable to this product.

NCBI GI #

169636420

NCBI GeneID

768

NCBI Accession #

NP_001207.2

UniProt Primary Accession #

Q16790

UniProt Secondary Accession #

Q5T4R1

UniProt Related Accession #

Q16790

GenBank Nucleotide #

NM_001216.2

NCBI Official Full Name

carbonic anhydrase 9

NCBI Official Synonym Full Names

carbonic anhydrase IX

NCBI Official Symbol

CA9

NCBI Official Synonym Symbols

MN; CAIX

UniProt Protein Name

Carbonic anhydrase 9

UniProt Synonym Protein Names

Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1

Protein Family

Carbonic anhydrase

UniProt Gene Name

CA9

UniProt Synonym Gene Names

G250; MN; CA-IX; CAIX; RCC-associated antigen G250

UniProt Entry Name

CAH9_HUMAN

NCBI Protein Information

carbonic anhydrase 9

NCBI Summary for CA9

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

UniProt Comments for CA9

CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family.

Protein type: Membrane protein, integral; Energy Metabolism - nitrogen; EC 4.2.1.1; Nucleolus; Lyase

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: microvillus membrane; basolateral plasma membrane; integral to membrane; nucleolus; plasma membrane

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: response to drug; secretion; bicarbonate transport; morphogenesis of an epithelium; response to testosterone stimulus; one-carbon compound metabolic process

Research Articles on CA9

1. High carbonic anhydrase IX expression in stromal cells is associated with invasiveness in gastric cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CA9 active protein

Reference Product	PubMed Publications
HIF1A active protein	>164 publications with CA9 and HIF1A
EGFR active protein	>28 publications with CA9 and EGFR
CTNNB1 active protein	>5 publications with CA9 and CTNNB1
TP53 active protein	>3 publications with CA9 and TP53
EP300 active protein	>3 publications with CA9 and EP300
ARNT active protein	>3 publications with CA9 and ARNT
SLC4A4 active protein	>2 publications with CA9 and SLC4A4
HIF3A active protein	>2 publications with CA9 and HIF3A

Diseases associated with CA9 active protein

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>254 publications with CA9 and Adenocarcinoma
Kidney Diseases Antibodies	>192 publications with CA9 and Kidney Diseases
Neoplasm Metastasis Antibodies	>136 publications with CA9 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>83 publications with CA9 and Breast Neoplasms
Anoxia Antibodies	>80 publications with CA9 and Anoxia
Neoplasms, Experimental Antibodies	>74 publications with CA9 and Neoplasms, Experimental
Necrosis Antibodies	>52 publications with CA9 and Necrosis
Lung Neoplasms Antibodies	>51 publications with CA9 and Lung Neoplasms
Neoplasm Invasiveness Antibodies	>50 publications with CA9 and Neoplasm Invasiveness
Nervous System Diseases Antibodies	>38 publications with CA9 and Nervous System Diseases

Organs/Tissues associated with CA9 active protein

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>201 publications with CA9 and Kidney
Blood Antibodies	>154 publications with CA9 and Blood
Lung Antibodies	>61 publications with CA9 and Lung
Brain Antibodies	>34 publications with CA9 and Brain
Cervix Antibodies	>30 publications with CA9 and Cervix
Bone Antibodies	>21 publications with CA9 and Bone
Pancreas Antibodies	>14 publications with CA9 and Pancreas
Stomach Antibodies	>13 publications with CA9 and Stomach
Intestine Antibodies	>10 publications with CA9 and Intestine
Uterus Antibodies	>9 publications with CA9 and Uterus