HAGH Active Protein

Name: HAGH Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS845364
Price: 600 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845364

Background: HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

HAGH, Active Protein

Full Product Name

HAGH, human recombinant

Product Gene Name

HAGH active protein

Product Synonym Names

Hydroxyacylglutathione hydrolase; GLO; GLX2; Glyoxalase II; HAGH1

Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD

Host

E Coli

Form/Format

0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Appearance: Liquid

Purity/Purification

>=95%

Concentration

0.5 mg/ml (lot specific)

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

31.4 kDa (284 aa, 1-260 aa + His Tag)

OMIM

138760

3D Structure

ModBase 3D Structure for Q16775

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is > 1.8 unit/ml, in which one unit will hydrolyze 1.0 umole of S-lactoylglutathione per minute at pH 7.5 at 25 degree C.

Results

Specific activity is > 1.8 unit/ml

Unit Definition

One unit will hydrolyze 1.0 umole of S-lactoylglutathione per minute at pH 7.5 at 25 degree C.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for HAGH active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

Applications Tested/Suitable for HAGH active protein

SDS-PAGE

Other Notes

Small volumes of HAGH active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HAGH purchase

MBS845364 is an active protein targeting HAGH. Also known as HAGH, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for HAGH. It may not necessarily be applicable to this product.

NCBI GI #

94538320

NCBI GeneID

3029

NCBI Accession #

NP_001035517.1

UniProt Primary Accession #

Q16775

UniProt Secondary Accession #

A8K290; B4DP33; B4DRA7; E7EN93

UniProt Related Accession #

Q16775

GenBank Nucleotide #

NM_001040427.1

NCBI Official Full Name

hydroxyacylglutathione hydrolase, mitochondrial isoform 2

NCBI Official Synonym Full Names

hydroxyacylglutathione hydrolase

NCBI Official Symbol

HAGH

NCBI Official Synonym Symbols

GLO2; GLX2; GLXII; HAGH1

UniProt Protein Name

Hydroxyacylglutathione hydrolase, mitochondrial

UniProt Synonym Protein Names

Glyoxalase II; Glx II

UniProt Gene Name

HAGH

UniProt Synonym Gene Names

GLO2; HAGH1; Glx II

UniProt Entry Name

GLO2_HUMAN

NCBI Protein Information

hydroxyacylglutathione hydrolase, mitochondrial

NCBI Summary for HAGH

The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

UniProt Comments for HAGH

HAGH: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid. Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.2.6; Hydrolase; Carbohydrate Metabolism - pyruvate; Mitochondrial

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrial matrix; cytoplasm

Molecular Function: hydroxyacylglutathione hydrolase activity; zinc ion binding

Biological Process: glutathione biosynthetic process

Disease: Hydroxyacyl Glutathione Hydrolase Deficiency

Research Articles on HAGH

1. No association between genetic variants of the HAGH gene and autism spectrum disorder was found.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HAGH active protein

Reference Product	PubMed Publications
PGP active protein	>4 publications with HAGH and PGP
GLO1 active protein	>4 publications with HAGH and GLO1
ESD active protein	>1 publications with HAGH and ESD

Pathways associated with HAGH active protein

Products by Pathway	Pathway Diagram
Pyruvate Metabolism Pathway antibodies	Pyruvate Metabolism Pathway Diagram
Pyruvate Metabolism Pathway antibodies	Pyruvate Metabolism Pathway Diagram

Diseases associated with HAGH active protein

Disease Name	Pubmed Publications
Congenital Abnormalities Antibodies	>2 publications with HAGH and Congenital Abnormalities
Heart Defects, Congenital Antibodies	>1 publications with HAGH and Heart Defects, Congenital
Infertility, Male Antibodies	>1 publications with HAGH and Infertility, Male
Heart Diseases Antibodies	>1 publications with HAGH and Heart Diseases
Kidney Diseases Antibodies	>1 publications with HAGH and Kidney Diseases
Inflammation Antibodies	>1 publications with HAGH and Inflammation

Organs/Tissues associated with HAGH active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>5 publications with HAGH and Brain
Kidney Antibodies	>3 publications with HAGH and Kidney
Skin Antibodies	>2 publications with HAGH and Skin
Heart Antibodies	>2 publications with HAGH and Heart
Blood Antibodies	>2 publications with HAGH and Blood
Nerve Antibodies	>1 publications with HAGH and Nerve
Muscle Antibodies	>1 publications with HAGH and Muscle
Lung Antibodies	>1 publications with HAGH and Lung