Kallikrein 8 (KLK8) Active Protein

Name: KLK8 Active Protein; Kallikrein 8; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS8120978
Price: 415 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8120978

Background: Kallikrein-8, also known as Neuropsin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein, and KLK8 is a secreted protein that belongs to the peptidase S1 family and Kallikrein subfamily. It is a… More details

$415 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Kallikrein 8 (KLK8), Active Protein

Full Product Name

Kallikrein 8 Protein, Human, Recombinant (His Tag)

Product Gene Name

KLK8 active protein

Product Synonym Names

Human KLK-8/Kallikrein-8 Protein (His Tag); HNP Protein, Human; NP Protein, Human; NRPN Protein, Human; PRSS19 Protein, Human; TADG14 Protein, Human; kallikrein-related peptidase 8

Sequence

Met1-Gly26 0

Host

HEK293 Cells

Form/Format

Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Purity/Purification

>98% as determined by SDS-PAGE

Preparation and Storage

Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.

Molecular Weight

28,048 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (51499264..51504958, complement). Location: 19q13

OMIM

605644

3D Structure

ModBase 3D Structure for O60259

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Species

Human

Endotoxin

<1.0EU per ug of the protein as determined by the LAL method

Predicted N Terminal

Gln 29

Tag

C-His

Protein Construction

A DNA sequence encoding the human KLK8 isoform 1 (O60259-1)(Met 1-Gly 260) was expressed, with a polyhistidine tag at the C-terminus.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Activity

Measured by its ability to cleave the fluorogenic peptide substrate BocVPRAMC. The specific activity is >400pmoles/min/ug

Product Categories/Family for KLK8 active protein

Serine Proteases and Regulators

Product References and Citations for KLK8 active protein

Yoshida S., et al.,(1998), Sequence analysis and expression of human neuropsin cDNA and gene. Gene 213:9-16.Underwood L.J., et al., (1999), Cloning of tumor-associated differentially expressed gene-14, a novel serine protease overexpressed by ovarian carcinoma.Cancer Res. 59:4435-4439.Mitsui S., et al.,(1999), A novel form of human neuropsin, a brain-related serine protease, is generated by alternative splicing and is expressed preferentially in human adult brain.Eur. J. Biochem. 260:627-634.

Other Notes

Small volumes of KLK8 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLK8 purchase

MBS8120978 is an active protein targeting KLK8. Also known as Kallikrein 8 Protein, Human, Recombinant (His Tag). Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for KLK8. It may not necessarily be applicable to this product.

NCBI GI #

6005844

NCBI GeneID

11202

NCBI Accession #

NP_009127.1

UniProt Primary Accession #

O60259

UniProt Secondary Accession #

Q5V9X1; Q5V9X2; Q8IW69; Q9HCB3; Q9NR68; Q9NR69; Q9UIL9; Q9UQ47

UniProt Related Accession #

O60259

GenBank Nucleotide #

NM_007196.2

NCBI Official Full Name

kallikrein-8 isoform 1 preproprotein

NCBI Official Synonym Full Names

kallikrein-related peptidase 8

NCBI Official Symbol

KLK8

NCBI Official Synonym Symbols

NP; HNP; NRPN; PRSS19; TADG14

UniProt Protein Name

Kallikrein-8

UniProt Synonym Protein Names

Neuropsin; NP; Ovasin; Serine protease 19; Serine protease TADG-14; Tumor-associated differentially expressed gene 14 protein

Protein Family

Kallikrein

UniProt Gene Name

KLK8

UniProt Synonym Gene Names

NRPN; PRSS19; TADG14; hK8; NP

UniProt Entry Name

KLK8_HUMAN

NCBI Protein Information

kallikrein-8; hK8; ovasin; neuropsin type 1; neuropsin type 2; serine protease 19; KLK8 protein type 1; KLK8 protein type 2; serine protease TADG-14; tumor-associated differentially expressed gene 14 protein

NCBI Summary for KLK8

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer. [provided by RefSeq, Jul 2008]

UniProt Comments for KLK8

KLK8: Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer- collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. Belongs to the peptidase S1 family. Kallikrein subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.4.21.118; Protease; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: extracellular space; cytoplasm

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: cell death; negative regulation of myelination; negative regulation of axon regeneration; synapse organization and biogenesis; response to wounding; regulation of synapse organization and biogenesis; keratinocyte proliferation; proteolysis; neurite morphogenesis; memory

Research Articles on KLK8

1. KLK8 and KLK14 can signal differentially via the PARs to affect tissue function

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with KLK8 active protein

Products by Pathway	Pathway Diagram
Spinal Cord Injury Pathway antibodies	Spinal Cord Injury Pathway Diagram

Diseases associated with KLK8 active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>25 publications with KLK8 and Neoplasms
Carcinoma Antibodies	>17 publications with KLK8 and Carcinoma
Adenocarcinoma Antibodies	>6 publications with KLK8 and Adenocarcinoma
Inflammation Antibodies	>5 publications with KLK8 and Inflammation
Breast Neoplasms Antibodies	>5 publications with KLK8 and Breast Neoplasms
Lung Neoplasms Antibodies	>4 publications with KLK8 and Lung Neoplasms
Seizures Antibodies	>2 publications with KLK8 and Seizures
Weight Gain Antibodies	>1 publications with KLK8 and Weight Gain
Stroke Antibodies	>1 publications with KLK8 and Stroke
Uterine Cervical Neoplasms Antibodies	>1 publications with KLK8 and Uterine Cervical Neoplasms

Organs/Tissues associated with KLK8 active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>44 publications with KLK8 and Brain
Skin Antibodies	>34 publications with KLK8 and Skin
Lung Antibodies	>5 publications with KLK8 and Lung
Esophagus Antibodies	>5 publications with KLK8 and Esophagus
Uterus Antibodies	>4 publications with KLK8 and Uterus
Placenta Antibodies	>2 publications with KLK8 and Placenta
Mammary Gland Antibodies	>2 publications with KLK8 and Mammary Gland
Liver Antibodies	>2 publications with KLK8 and Liver
Lymph Node Antibodies	>1 publications with KLK8 and Lymph Node
Stomach Antibodies	>1 publications with KLK8 and Stomach