• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Exonuclease I (EXO1) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845597

Exonuclease I hydrolyzes single-stranded DNA in the 3'-->5' direction; releasing 5'-mononucleotides and leaving the terminal 5'-dinucleotide intact. Hydrolysis is processive and cannot proceed if the 3' terminus is phosphorylated. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
Exonuclease I (Exo1), Active Protein
Full Product Name
Exonuclease I
Product Gene Name
Exo1 active protein
Product Synonym Names
Exo I
Host
E Coli strain containing an overproducing clone of E Coli Exonuclease I.
Form/Format
Liquid
Exonuclease I in 20 mM Tris-HCI (pH 7.5); 5 mM 2-mercaptoethanol; 0.5 mM EDTA; 50% glycerol.
Purity/Purification
>=95%
Preparation and Storage
Store at -20 degree C for 12 months.
Centrifuge the vial prior to opening.
Molecular Weight
55.0 kDa
3D Structure
ModBase 3D Structure for Q9QZ11
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Unit Definition
One unit is the amount of enzyme which catalyzes the release of 10 nmol of acid-soluble nucleotide from denatured DNA in 30 min at 37 degree C under standard conditions.
Activity
10 unit/mul
Applications Tested/Suitable for Exo1 active protein
SDS-PAGE

Other Notes

Small volumes of Exo1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Exo1 purchase

MBS845597 is an active protein targeting Exo1. Also known as Exonuclease I. Host: E Coli strain containing an overproducing clone of E Coli Exonuclease I.

Additional Details

NCBI/Uniprot data below describe general gene information for Exo1. It may not necessarily be applicable to this product.

NCBI GI #
31560511
NCBI GeneID
26909
NCBI Accession #
NP_036142.2
UniProt Primary Accession #
Q9QZ11
UniProt Secondary Accession #
Q3TLM4; Q923A5
UniProt Related Accession #
Q9QZ11
GenBank Nucleotide #
NM_012012.4
NCBI Official Full Name
exonuclease 1
NCBI Official Synonym Full Names
exonuclease 1
NCBI Official Symbol
Exo1
NCBI Official Synonym Symbols
Msa; 5730442G03Rik
UniProt Protein Name
Exonuclease 1
UniProt Synonym Protein Names
Exonuclease I
Protein Family
Exodeoxyribonuclease
UniProt Gene Name
Exo1
UniProt Synonym Gene Names
mExo1
NCBI Protein Information
exonuclease 1

UniProt Comments for Exo1

EXO1: 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.-.-; Hydrolase

Chromosomal Location of Human Ortholog: 1 H4|1 81.9 cM

Cellular Component: nuclear body; nucleoplasm; nucleus

Molecular Function: 5'-3' exodeoxyribonuclease activity; 5'-3' exonuclease activity; chromatin binding; DNA binding; double-stranded DNA specific 5'-3' exodeoxyribonuclease activity; flap endonuclease activity; single-stranded DNA specific 5'-3' exodeoxyribonuclease activity

Biological Process: DNA recombination; humoral immune response mediated by circulating immunoglobulin; isotype switching; mismatch repair; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; somatic hypermutation of immunoglobulin genes
Show more

Research Articles on Exo1

1. The results establish a key role for EXO1 in modulating the severity of hypomorphic MRE11 complex mutations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Exo1 active protein

 Products by Pathway  Pathway Diagram
 Mismatch Repair Pathway antibodies  Mismatch Repair Pathway Diagram
 Mismatch Repair Pathway antibodies  Mismatch Repair Pathway Diagram
 Mismatch Repair Pathway antibodies  Mismatch Repair Pathway Diagram

Diseases associated with Exo1 active protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >47 publications with Exo1 and Neoplasms
 Gastrointestinal Diseases Antibodies  >20 publications with Exo1 and Gastrointestinal Diseases
 Colorectal Neoplasms Antibodies  >19 publications with Exo1 and Colorectal Neoplasms
 Liver Neoplasms Antibodies  >5 publications with Exo1 and Liver Neoplasms
 Nervous System Diseases Antibodies  >5 publications with Exo1 and Nervous System Diseases
 Liver Diseases Antibodies  >4 publications with Exo1 and Liver Diseases
 Neoplasms, Experimental Antibodies  >4 publications with Exo1 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >3 publications with Exo1 and Breast Neoplasms
 Brain Diseases Antibodies  >3 publications with Exo1 and Brain Diseases
 Congenital Abnormalities Antibodies  >3 publications with Exo1 and Congenital Abnormalities

Organs/Tissues associated with Exo1 active protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >7 publications with Exo1 and Liver
 Testis Antibodies  >7 publications with Exo1 and Testis
 Lung Antibodies  >7 publications with Exo1 and Lung
 Brain Antibodies  >6 publications with Exo1 and Brain
 Bone Antibodies  >5 publications with Exo1 and Bone
 Thymus Antibodies  >3 publications with Exo1 and Thymus
 Bone Marrow Antibodies  >3 publications with Exo1 and Bone Marrow
 Kidney Antibodies  >3 publications with Exo1 and Kidney
 Stomach Antibodies  >3 publications with Exo1 and Stomach
 Skin Antibodies  >1 publications with Exo1 and Skin
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000