Mouse Anti-CAS9 Monoclonal Antibody – Cat# MBS845947

CAS9 Antibody – Mouse Monoclonal

Name: CAS9 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS845947
Price: 640 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845947

Monoclonal antibody to detect Cas9.Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes and other… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Cas9, Monoclonal Antibody

Full Product Name

Cas9 Monoclonal Antibody

Product Gene Name

anti-Cas9 antibody

Product Synonym Names

CRISPR-associated endonuclease Cas9/Csn1; CRISPR associated protein 9; SpyCas9

Clonality

Monoclonal

Isotype

IgG1 kappa

Clone Number

7A9

Host

Mouse

Form/Format

Colorless liquid
PBS containing 0.02% sodium azide.

Purity/Purification

Affinity purified using protein G

Preparation and Storage

Store at -20 degree C for 12 months.
The antibody solution should be gently mixed before use.

Molecular Weight

158,441 Da

3D Structure

ModBase 3D Structure for Q99ZW2

Immunogen

N-terminal fragment of Streptococcus pyogenes Cas9.

Product Categories/Family for anti-Cas9 antibody

Epigenetic

Applications Tested/Suitable for anti-Cas9 antibody

Immunofluorescence (IF); Immunoprecipitation (IP); Western Blot (WB)

Application Notes for anti-Cas9 antibody

IF: 1:500-1:2; 000
IP: 1:20
WB: 1:1; 000

Other Notes

Small volumes of anti-Cas9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cas9 purchase

MBS845947 is an antibody targeting Cas9. Also known as Cas9 Monoclonal Antibody. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Cas9. It may not necessarily be applicable to this product.

NCBI GI #

15675041

NCBI GeneID

901176

NCBI Accession #

NP_269215.1

UniProt Primary Accession #

Q99ZW2

GenBank Nucleotide #

NC_002737.2

NCBI Official Full Name

conserved hypothetical protein

NCBI Official Symbol

SPy_1046

UniProt Protein Name

CRISPR-associated endonuclease Cas9/Csn1

UniProt Synonym Protein Names

SpCas9

Protein Family

CRISPR-associated endonuclease

UniProt Gene Name

cas9

NCBI Protein Information

hypothetical protein

UniProt Comments for Cas9

Cas9: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA probably requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA (By similarity). Cas9/crRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. Binding to dsDNA target requires a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif). When the CRISPR3/cas system consisting of cas9-cas1-cas2-csn2-CRISPR3 or just cas9-CRISPR3 is expressed in E.coli it prevents plasmids homologous to spacers 1 or 2 from transforming

Protein type: EC 3.1.-.-

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.