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His-Tag Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS846016

Rabbit monoclonal antibody to detect His-Tag.A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues; followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
His-Tag, Monoclonal Antibody
Full Product Name
Anti-His-Tag Rabbit Monoclonal Antibody
Clonality
Monoclonal
Isotype
IgG
Clone Number
RM146
Host
Rabbit
Form/Format
Colorless liquid
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Purity/Purification
Affinity purified using protein A
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C for 12 months.
The antibody solution should be gently mixed before use.
Immunogen
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Applications Tested/Suitable for anti-His-Tag antibody
Immunoprecipitation (IP); Western Blot (WB); Immunocytochemistry (ICC); Flow Cytometry (FC/FACS); Immunohistochemistry (IHC)
Application Notes for anti-His-Tag antibody
WB: 0.1 ug/mL- 1 ug/mL
IP: 0.5 ug/mL- 2 ug/mL
ICC: 0.5 ug/mL- 2 ug/mL
FC: 0.5 ug/mL- 2 ug/mL
IHC: 0.1 ug/mL-1 ug/mL.

Other Notes

Small volumes of anti-His-Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for His-Tag purchase

MBS846016 is an antibody targeting His-Tag. Also known as Anti-His-Tag Rabbit Monoclonal Antibody. Host: Rabbit.
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