E Coli Recombinant CAN Active Protein – Cat# MBS846977

Carbonic Anhydrase (CAN) E Coli Recombinant Active Protein

Name: CAN Active Protein; Carbonic Anhydrase; E Coli Recombinant; MyBioSource
Brand: MyBioSource
SKU: MBS846977
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS846977

Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O HCO3- + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Carbonic anhydrase (CAN), Active Protein

Full Product Name

E Coli Recombinant Carbonic anhydrase

Product Gene Name

CAN active protein

Product Synonym Names

Carbonate dehydratase; CAN; yadF

Sequence

MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK

Host

E Coli

Form/Format

Liquid
In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT; 10% glycerol

Purity/Purification

> 95%

Preparation and Storage

Store at -20 degree C for 12 months.
Centrifuge the vial prior to opening.

Molecular Weight

27 kDa

3D Structure

ModBase 3D Structure for P61517

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Results

>1,000 pmol/min/ug

Applications Tested/Suitable for CAN active protein

SDS-PAGE

Other Notes

Small volumes of CAN active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CAN purchase

MBS846977 is an active protein targeting CAN. Also known as E Coli Recombinant Carbonic anhydrase. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CAN. It may not necessarily be applicable to this product.

NCBI GI #

16128119

NCBI GeneID

944832

NCBI Accession #

NP_414668.1

UniProt Primary Accession #

P61517

UniProt Secondary Accession #

P36857; P75656; Q8KJQ4

GenBank Nucleotide #

NC_000913.3

NCBI Official Full Name

carbonic anhydrase

NCBI Official Symbol

can

NCBI Official Synonym Symbols

ECK0125; JW0122; yadF

UniProt Protein Name

Carbonic anhydrase 2

UniProt Synonym Protein Names

Carbonate dehydratase 2

Protein Family

Carbonic anhydrase

UniProt Gene Name

can

UniProt Synonym Gene Names

cynT2; yadF

NCBI Protein Information

carbonic anhydrase

NCBI Summary for CAN

can forms a synthetic lethal with cynT, rescued by high CO2. [More information is available at EcoGene: EG12319]. Escherichia coli encodes two carbonic anhydrases, Can (carbonic anhydrase 2) and CynT (carbonic anhydrase 1). [More information is available at EcoCyc: EG12319].

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CAN active protein

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Nitrogen Metabolism Pathway antibodies	Nitrogen Metabolism Pathway Diagram
Nitrogen Metabolism Pathway antibodies	Nitrogen Metabolism Pathway Diagram