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Oxalate Oxidase (OxOx) B. Subtilis Recombinant Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS847019

Oxalate oxidase is a member of the cupin superfamily. It catalyzes the breakdown of oxalate to hydrogen peroxide and carbon dioxide. Oxalate oxidase and oxalate decarboxylase are two similar enzymes that share similar structural and sequence identity. Both… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Oxalate oxidase (OxOx), Active Protein
Full Product Name
B. subtilis Recombinant; Oxalate oxidase (OxOx)
Product Gene Name
OxOx active protein
Product Synonym Gene Name
oxdC
Product Synonym Names
OxO; OxOx; OxO_r
Sequence
Refer to B. subtilis oxalate decarboxyl ase. Oxalate oxidase is derived from oxalate decarboxyl ase by introducin g three mutations: S161D, E162A, and N163S
Host
E Coli
Source: Bacillus subtilis
Form/Format
Freeze-dried from proprietary buffer
Purity/Purification
>=90%
Preparation and Storage
Store at -20 degree C. Stable for at least 2 years as supplied.
Molecular Weight
43.6 kDa
3D Structure
ModBase 3D Structure for O34714
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Tag Information
His-tagged
Reconstitution
Reconstitute to 2 mg/mL in sterile water, store at -80 degree C in aliquots and use within 6 months after reconstitution. Avoid repeated freeze-thaw cycles.
Specific Activity
The enzyme has a specific activity of > = 230 mU/mg based on measuring the production of H2O2 from the following reaction:
(COOH)2(Oxalate) + O2 --> 2CO2 + H2O2
Unit Definition
One unit is the amount of enzyme that converts 1 umole of oxalate to CO2 and H2O2 per minute at pH 4.5 and 25 degree C.
Applications Tested/Suitable for OxOx active protein
SDS-PAGE

SDS-PAGE (SDS) of OxOx active protein

SDS-PAGE (4-20%) of Recombinant OxOx: Recombinant Protein loaded under reducing conditions and stained with Coomassie Blue. The protein shows a predicted MW of ~ 43.6 kDa
B. Subtilis Recombinant OxOx Active Protein – Cat# MBS847019

Test Data of OxOx active protein

OxOx activity assay: Specific activity of oxalate oxidase is > = 230 mU/m. Different amount of enzyme reacted with 1.36 mM of oxalate yield the different rates. The specific activity is calculated by the decrease in absorbance at 570 nm after 10 min incubation at 25 degree C as measured by Oxalate Oxidase
B. Subtilis Recombinant OxOx Active Protein, Test Data – Cat# MBS847019

Other Notes

Small volumes of OxOx active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OxOx purchase

MBS847019 is an active protein targeting OxOx. Also known as B. subtilis Recombinant; Oxalate oxidase (OxOx). Host: E Coli
Source: Bacillus subtilis.

Additional Details

NCBI/Uniprot data below describe general gene information for OxOx. It may not necessarily be applicable to this product.

NCBI GI #
16080377
NCBI GeneID
938620
NCBI Accession #
NP_391204.1
UniProt Primary Accession #
O34714
UniProt Related Accession #
O34714
GenBank Nucleotide #
NC_000964.3
NCBI Official Full Name
oxalate decarboxylase OxdC
NCBI Official Symbol
oxdC
UniProt Protein Name
Oxalate decarboxylase OxdC
UniProt Gene Name
oxdC
NCBI Protein Information
oxalate decarboxylase OxdC

UniProt Comments for OxOx

Converts oxalate to formate and CO2 in an O2-dependent reaction. Can also catalyze minor side reactions: oxalate oxidation to produce H2O2, and oxalate-dependent, H2O2-independent dye oxidations.

Research Articles on OxOx

1. The pH dependence of the Mn(III) electron paramagnetic resonance (EPR) signal compares very well with that of enzymatic activity, providing evidence that the catalytic reaction of oxalate decarboxylase is driven by Mn(III), which is generated in the presence of dioxygen.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with OxOx active protein

 Reference Product  PubMed Publications
 yvrL active protein  >2 publications with OxOx and yvrL
 yvrJ active protein  >2 publications with OxOx and yvrJ

Pathways associated with OxOx active protein

 Products by Pathway  Pathway Diagram
 Glyoxylate And Dicarboxylate Metabolism Pathway antibodies  Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
 Glyoxylate And Dicarboxylate Metabolism Pathway antibodies  Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Oxalate Degradation V Pathway antibodies  Oxalate Degradation V Pathway Diagram
 Oxalate Degradation V Pathway antibodies  Oxalate Degradation V Pathway Diagram
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