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GPT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS847145

GPT (Glutamate-pyruvate transaminase), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. This protein has 2 distinct molecular and genetic forms: one cytoplasmic… More details

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Product Overview

Product Name
GPT/ALT1, Polyclonal Antibody
Full Product Name
Anti-GPT/ALT1 Antibody
Product Gene Name
Product Synonym Names
Alanine aminotransferase 1; ALT1; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; GPT; AAT1; GPT1
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Colorless liquid. In PBS with 0.09% (W/V) sodium azide
Purity/Purification
Saturated Ammonium Sulfate (SAS)precipitation followed by dialysis against PBS.
Specificity
Polyclonal antibody to detect endogenous levels of GPT/ALT1 in human samples.
Preparation and Storage
Store at -20 degree C
The antibody solution should be gently mixed before use
Shelf life: ~12 months
Molecular Weight
54,637 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (145729465..145732555). Location: 8q24.3
OMIM
138200
3D Structure
ModBase 3D Structure for P24298
Immunogen
KLH conjugated synthetic peptide between 118-144 amino acids from the N-terminal region of human GPT
Positive Control
IHC: Human liver and kidney tissue sections; WB: U87-MG cell line, mouse liver and rat heart and liver tissue lysates
Applications Tested/Suitable for anti-GPT antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-GPT antibody
IHC: 1:100
WB: 1:1000

Testing Data of anti-GPT antibody

Rabbit Anti-GPT Polyclonal Antibody – Cat# MBS847145

Other Notes

Small volumes of anti-GPT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPT purchase

MBS847145 is an antibody targeting GPT. Also known as Anti-GPT/ALT1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GPT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
alanine aminotransferase
NCBI Official Synonym Full Names
glutamic-pyruvate transaminase (alanine aminotransferase)
NCBI Official Symbol
GPT
NCBI Official Synonym Symbols
AAT1; ALT1; GPT1
UniProt Protein Name
Alanine aminotransferase 1
UniProt Synonym Protein Names
Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
UniProt Gene Name
GPT
UniProt Synonym Gene Names
AAT1; GPT1; ALT1; GPT 1
UniProt Entry Name
ALAT1_HUMAN
NCBI Protein Information
alanine aminotransferase 1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1

NCBI Summary for GPT

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
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UniProt Comments for GPT

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2; Transferase

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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