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5-Methylcytosine (5-mc) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS847167

5-methylcytosine (5-mC) is a modified base present in nucleic acids of plants and vertebrates. DNA methylation is a post-replicative process involved in the establishment of genomic imprinting, in the control of gene expression and of differentiation.… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
5-Methylcytosine (5-mc), Monoclonal Antibody
Full Product Name
Anti-5-Methylcytosine Antibody
Product Gene Name
Product Synonym Names
5mc
Clonality
Monoclonal
Isotype
Mouse IgG1, lambda
Clone Number
33D3
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Colorless liquid. Phosphate Buffer 10mM - NaCl 0.15 M - pH7.4
Purity/Purification
Affinity purification. Purified IgG fraction prepared by affinity chromatography on protein A
Specificity
Monoclonal antibody to detect 5-Methylcytosine
Preparation and Storage
Store at -20 degree C
The antibody solution should be gently mixed before use
Shelf life: ~12 months
Immunogen
Modified ribonucleoside, specific for the presence of a methyl group on carbon 5 of the pyrimidine ring
Applications Tested/Suitable for anti-5-mc antibody
Western Blot (WB), ELISA (EIA), Methylated DNA Immunopreciptation (MeDIP), Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF),, Immunohistochemistry (IHC), FC
Application Notes for anti-5-mc antibody
WB: 1:250
ELISA: 1:10,000
MeDIP: 1-2ug per IP
ChIP: 1-2ug per IP
IF: 1:500
IHC Perform heat mediated antigen retrieval before commencing with IHC staining protocol
ICC: 0.5-5ug/ml
FC: 10ul of working dilution to label 10 x 106 cells in 100ul

Other Notes

Small volumes of anti-5-mc antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 5-mc purchase

MBS847167 is an antibody targeting 5-mc. Also known as Anti-5-Methylcytosine Antibody. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for 5-mc. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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