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CD50 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS847405

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
CD50/ICAM-3, Monoclonal Antibody
Full Product Name
Anti-CD50/ICAM-3 Antibody (Clone 186-2G9)
Product Gene Name
Product Synonym Gene Name
ICAM-3, ICAM
Product Synonym Names
Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD50; ICAMR
Clonality
Monoclonal
Isotype
Mouse IgG2b, kappa
Clone Number
186-2G9
Host
Mouse
Species Reactivity
Human
Form/Format
Colorless liquid. In 10mM PBS with 0.05% BSA & 0.05% azide
Purity/Purification
Protein A/G purification
Specificity
Monoclonal Antibody to detect CD50/ICAM-3 in human samples
Preparation and Storage
Store at -20 degree C
The antibody solution should be gently mixed before use
Shelf life: ~12 months
Molecular Weight
59,541 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (10444452..10450345, complement). Location: 19p13.3-p13.2
OMIM
146631
3D Structure
ModBase 3D Structure for P32942
Immunogen
Stimulated human leukocytes
Positive Control
IHC: Human Tonsil
Applications Tested/Suitable for anti-CD50 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-CD50 antibody
FC/FACS: 0.5-1ug/1x106 cells in 0.1 ml
IF: 0.5-1ug/ml
IHC: 0.5-1ug/ml for 30 minutes at room temperature

Immunohistochemistry (IHC) of anti-CD50 antibody

Mouse Anti-CD50 Monoclonal Antibody – Cat# MBS847405

Other Notes

Small volumes of anti-CD50 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD50 purchase

MBS847405 is an antibody targeting CD50. Also known as Anti-CD50/ICAM-3 Antibody (Clone 186-2G9). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD50. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
intercellular adhesion molecule 3
NCBI Official Synonym Full Names
intercellular adhesion molecule 3
NCBI Official Symbol
ICAM3
NCBI Official Synonym Symbols
CD50; CDW50; ICAM-R
UniProt Protein Name
Intercellular adhesion molecule 3
UniProt Synonym Protein Names
CDw50; ICAM-R
UniProt Gene Name
ICAM3
UniProt Synonym Gene Names
ICAM-3
UniProt Entry Name
ICAM3_HUMAN
NCBI Protein Information
intercellular adhesion molecule 3; ICAM-3; intercellular adhesion molecule-3

NCBI Summary for CD50

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD50

ICAM3: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. Belongs to the immunoglobulin superfamily. ICAM family.

Protein type: Cell adhesion; Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: integrin binding; protein binding; receptor binding

Biological Process: cell-cell adhesion; extracellular matrix organization and biogenesis; regulation of immune response; cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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