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RAB25 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS850064

Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The… More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Rab25, Antibody
Full Product Name
Rab25 Antibody
Product Gene Name
Product Synonym Names
CATX-8; RAB25
Isotype
IgG2a
Clone Number
3F12F3
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Purified antibody in PBS containing 0.03% sodium azide.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Molecular Weight
23,496 Da
OMIM
612942
3D Structure
ModBase 3D Structure for P57735
Immunogen
Purified recombinant fragment of Rab25 expressed in E. Coli
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-Rab25 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-Rab25 antibody
WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-Rab25 antibody

Figure 1. Western blot analysis using Rab25 mouse mAb against truncated Rab25 recombinant protein (1), human overy carcinoma(2), stomach carcinoma (3), breast carcinoma (4) tissue lysate.
Mouse Anti-RAB25 Antibody – Cat# MBS850064

Immunohistochemistry (IHC) of anti-Rab25 antibody

Figure 2. Immunohistochemical analysis of paraffin-embedded human bladder carcinoma (A), breast carcinoma (B), esophagus carcinoma (C), skin carcinoma (D) tissue, showing cytoplasmic localization using Rab25 mouse mAb with DAB staining.
Mouse Anti-RAB25 Antibody, Immunohistochemistry (IHC) – Cat# MBS850064

Other Notes

Small volumes of anti-Rab25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rab25 purchase

MBS850064 is an antibody targeting Rab25. Also known as Rab25 Antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Rab25. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ras-related protein Rab-25
NCBI Official Synonym Full Names
RAB25, member RAS oncogene family
NCBI Official Symbol
RAB25
NCBI Official Synonym Symbols
CATX-8; RAB11C
UniProt Protein Name
Ras-related protein Rab-25
UniProt Synonym Protein Names
CATX-8
Protein Family
UniProt Gene Name
RAB25
NCBI Protein Information
ras-related protein Rab-25

NCBI Summary for Rab25

The protein encoded by this gene is a member of the RAS superfamily of small GTPases. The encoded protein is involved in membrane trafficking and cell survival. This gene has been found to be a tumor suppressor and an oncogene, depending on the context. Two variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq, Nov 2015]
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UniProt Comments for Rab25

Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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