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KLHL22 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS850207

KLHL22 (kelch-like protein 22) is a 634 amino acid protein that is related to the Drosophilakelch protein, which is required to maintain Actin organization in ovarian ring canals. Mutations affecting Kelch function result in the failure of Kelch to associate… More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KLHL22, Antibody
Full Product Name
KLHL22 Antibody
Product Gene Name
anti-KLHL22 antibody
Product Synonym Names
KELCHL; KLHL22
Isotype
IgG1
Clone Number
7B10
Host
Mouse
Species Reactivity
Human
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.
Molecular Weight
72kDa
3D Structure
ModBase 3D Structure for Q53GT1
Immunogen
Purified recombinant fragment of human KLHL22 expressed in E. Coli.

Product Categories/Family for anti-KLHL22 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-KLHL22 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-KLHL22 antibody
WB: 1:500-1:2000
IF: 1:200-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-KLHL22 antibody

Figure 1: Western blot analysis using KLHL22 mouse mAb against mouse brain tissues lysate.
Mouse Anti-KLHL22 Antibody – Cat# MBS850207

Immunofluorescence (IF) of anti-KLHL22 antibody

Figure 2: Immunofluorescence analysis of U251 cells using KLHL22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Mouse Anti-KLHL22 Antibody, Immunofluorescence (IF) – Cat# MBS850207

Other Notes

Small volumes of anti-KLHL22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLHL22 purchase

MBS850207 is an antibody targeting KLHL22. Also known as KLHL22 Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KLHL22. It may not necessarily be applicable to this product.

NCBI GI #
24432026
NCBI GeneID
84861
NCBI Accession #
NP_116164.2
UniProt Primary Accession #
Q53GT1
UniProt Secondary Accession #
Q96B68; Q96KC6; A8K3Q4; A8MTV3; B7Z2G1; D3DX30
UniProt Related Accession #
Q53GT1
GenBank Nucleotide #
NM_032775.3
NCBI Official Full Name
kelch-like protein 22
NCBI Official Synonym Full Names
kelch like family member 22
NCBI Official Symbol
KLHL22
NCBI Official Synonym Symbols
KELCHL
UniProt Protein Name
Kelch-like protein 22
Protein Family
Kelch-like protein
UniProt Gene Name
KLHL22
NCBI Protein Information
kelch-like protein 22

UniProt Comments for KLHL22

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation.
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Research Articles on KLHL22

1. CUL3/KLHL22 may contact two distinct motifs within PLK1 protein, consistent with the bivalent mode of substrate targeting.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KLHL22 antibody

 Reference Product  PubMed Publications
 CUL3 antibody  >4 publications with KLHL22 and CUL3
 KLHL9 antibody  >1 publications with KLHL22 and KLHL9
 KLHL21 antibody  >1 publications with KLHL22 and KLHL21
 KLHL13 antibody  >1 publications with KLHL22 and KLHL13

Diseases associated with anti-KLHL22 antibody

 Disease Name  Pubmed Publications
 Liver Neoplasms Antibodies  >1 publications with KLHL22 and Liver Neoplasms

Organs/Tissues associated with anti-KLHL22 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1 publications with KLHL22 and Liver
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