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Cytokeratin (KRT5) Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS850272

Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin (KRT5), Antibody
Full Product Name
Cytokeratin (Pan) Antibody
Product Gene Name
Product Synonym Names
K5; DDD; EBS2; KRT5A; KRT5
Isotype
IgG1
Clone Number
7H8
Host
Mouse
Species Reactivity
Human
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Molecular Weight
62,378 Da
OMIM
131760
3D Structure
ModBase 3D Structure for P13647
Immunogen
Purified recombinant fragment of Cytokeratin 5 expressed in E. Coli.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-KRT5 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-KRT5 antibody
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000

Immunohistochemistry (IHC) of anti-KRT5 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma (A), normal hepatocyte (B), colon adenocacinoma, normal stomach tissue (D), showing cytoplasmic and membrane localization using CK mouse mAb with DAB staining.
Mouse Anti-KRT5 Antibody – Cat# MBS850272

Immunofluorescence (IF) of anti-KRT5 antibody

Figure 2. Confocal immunofluorescence analysis of methanol-fixed Eca-109 cells using Cytokeratin (Pan) mouse mAb (green), showing cytoplasmic localization. Blue. DRAQ5 fluorescent DNA dye.
Mouse Anti-KRT5 Antibody, Immunofluorescence (IF) – Cat# MBS850272

Other Notes

Small volumes of anti-KRT5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT5 purchase

MBS850272 is an antibody targeting KRT5. Also known as Cytokeratin (Pan) Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type II cytoskeletal 5
NCBI Official Synonym Full Names
keratin 5
NCBI Official Symbol
KRT5
NCBI Official Synonym Symbols
K5; CK5; DDD; DDD1; EBS2; KRT5A
UniProt Protein Name
Keratin, type II cytoskeletal 5
UniProt Synonym Protein Names
58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5
Protein Family
UniProt Gene Name
KRT5
UniProt Synonym Gene Names
CK-5; K5
NCBI Protein Information
keratin, type II cytoskeletal 5

NCBI Summary for KRT5

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT5

MiscellaneousThere are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT5 antibody

Pathways associated with anti-KRT5 antibody

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