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PDK1 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS850452

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PDK1, Antibody
Full Product Name
PDK1 Antibody
Product Gene Name
Product Synonym Names
PDK1
Isotype
IgG1
Clone Number
4A11
Host
Mouse
Species Reactivity
Human, Monkey, Rat
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Preparation and Storage
Store at 4°C, for long term storage, store at -20°C.
Molecular Weight
44kDa
OMIM
602524
3D Structure
ModBase 3D Structure for Q15118
Immunogen
Purified recombinant fragment of human PDK1 expressed in E. Coli.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PDK1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-PDK1 antibody
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000

Western Blot (WB) of anti-PDK1 antibody

Figure 1: Western blot analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.
Mouse Anti-PDK1 Antibody – Cat# MBS850452

Immunohistochemistry (IHC) of anti-PDK1 antibody

Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.
Mouse Anti-PDK1 Antibody, Immunohistochemistry (IHC) – Cat# MBS850452

Immunofluorescence (IF) of anti-PDK1 antibody

Figure 3: Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Mouse Anti-PDK1 Antibody, Immunofluorescence (IF) – Cat# MBS850452

Other Notes

Small volumes of anti-PDK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDK1 purchase

MBS850452 is an antibody targeting PDK1. Also known as PDK1 Antibody. Reactive in Human, Monkey, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PDK1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial isoform 2
NCBI Official Synonym Full Names
pyruvate dehydrogenase kinase 1
NCBI Official Symbol
PDK1
UniProt Protein Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
UniProt Synonym Protein Names
Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1
UniProt Gene Name
PDK1
UniProt Synonym Gene Names
PDHK1; PDH kinase 1
NCBI Protein Information
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial

NCBI Summary for PDK1

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]
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UniProt Comments for PDK1

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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