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HK2 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS850501

The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HK2, Antibody
Full Product Name
HK2 Antibody
Product Gene Name
Product Synonym Names
HKII; HXK2; DKFZp686M1669; HK2
Isotype
IgG1
Clone Number
3D3
Host
Mouse
Species Reactivity
Human
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.
Molecular Weight
102kDa
OMIM
601125
3D Structure
ModBase 3D Structure for P52789
Immunogen
Purified recombinant fragment of human HK2 expressed in E. Coli.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-HK2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-HK2 antibody
WB: 1:500-1:2000
IHC: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000

Western Blot of anti-HK2 antibody

Western blot analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.
Mouse Anti-HK2 Antibody – Cat# MBS850501

Immunohistochemistry of anti-HK2 antibody

Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb with DAB staining.
Mouse Anti-HK2 Antibody, Immunohistochemistry – Cat# MBS850501

Flow Cytometry of anti-HK2 antibody

Flow cytometric analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).
Mouse Anti-HK2 Antibody, Flow Cytometry – Cat# MBS850501

Other Notes

Small volumes of anti-HK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HK2 purchase

MBS850501 is an antibody targeting HK2. Also known as HK2 Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
hexokinase-2
NCBI Official Synonym Full Names
hexokinase 2
NCBI Official Symbol
HK2
NCBI Official Synonym Symbols
HKII; HXK2
UniProt Protein Name
Hexokinase-2
UniProt Synonym Protein Names
Hexokinase type II; HK II; Muscle form hexokinase
UniProt Gene Name
HK2
UniProt Synonym Gene Names
HK II
NCBI Protein Information
hexokinase-2

NCBI Summary for HK2

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]
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UniProt Comments for HK2

MiscellaneousIn vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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