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Metadherin (MTDH) Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS850543

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Metadherin (MTDH), Antibody
Full Product Name
Metadherin Antibody
Product Gene Name
Product Synonym Names
3D3; AEG1; LYRIC
Isotype
IgG1
Clone Number
2F11C3
Host
Mouse
Species Reactivity
Human
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Preparation and Storage
Store at 4°C, for long term storage, store at -20°C
Molecular Weight
64kDa
OMIM
610323
3D Structure
ModBase 3D Structure for Q86UE4
Immunogen
Purified recombinant fragment of human Metadherin expressed in E. Coli.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MTDH antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-MTDH antibody
WB: 1:500-1:2000
IHC: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Western Blot (WB) of anti-MTDH antibody

Figure 1. Western blot analysis using Metadherin mouse mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.
Mouse Anti-MTDH Antibody – Cat# MBS850543

Immunohistochemistry (IHC) of anti-MTDH antibody

Figure 2. Immunohistochemical analysis of paraffin-embedded human Liver tissues using Metadherin mouse mAb
Mouse Anti-MTDH Antibody, Immunohistochemistry (IHC) – Cat# MBS850543

Flow Cytometry (FC/FACS) of anti-MTDH antibody

Figure 3. Flow cytometric analysis of Hela cells using Metadherin mouse mAb (green) and negative control (purple).
Mouse Anti-MTDH Antibody, Flow Cytometry (FC/FACS) – Cat# MBS850543

Other Notes

Small volumes of anti-MTDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MTDH purchase

MBS850543 is an antibody targeting MTDH. Also known as Metadherin Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MTDH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein LYRIC isoform 1
NCBI Official Synonym Full Names
metadherin
NCBI Official Symbol
MTDH
NCBI Official Synonym Symbols
3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
UniProt Protein Name
Protein LYRIC
UniProt Synonym Protein Names
3D3/LYRIC; Astrocyte elevated gene-1 protein; AEG-1; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein
Protein Family
UniProt Gene Name
MTDH
UniProt Synonym Gene Names
AEG1; LYRIC; AEG-1
NCBI Protein Information
protein LYRIC

UniProt Comments for MTDH

Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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