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MLH1 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS850685

DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different… More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MLH1, Antibody
Full Product Name
MLH1 Antibody
Product Gene Name
anti-MLH1 antibody
Product Synonym Names
FCC2; COCA2; HNPCC
Isotype
IgG1
Clone Number
4C9C7
Host
Mouse
Species Reactivity
Human, Monkey
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Molecular Weight
85kDa
OMIM
600050
3D Structure
ModBase 3D Structure for Q16584
Immunogen
Purified recombinant fragment of ABL1 (aa577-650) expressed in E. Coli.

Product Categories/Family for anti-MLH1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MLH1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-MLH1 antibody
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-MLH1 antibody

Figure 1. Western blot analysis using MLH1 mouse mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.
Mouse Anti-MLH1 Antibody – Cat# MBS850685

Immunohistochemistry (IHC) of anti-MLH1 antibody

Figure 2. Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 mouse mAb.
Mouse Anti-MLH1 Antibody, Immunohistochemistry (IHC) – Cat# MBS850685

Immunofluorescence (IF) of anti-MLH1 antibody

Figure 3. Confocal immunofluorescence analysis of Hela cells using MLH1 mouse mAb (green), showing nuclear localization. Red. Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Mouse Anti-MLH1 Antibody, Immunofluorescence (IF) – Cat# MBS850685

Other Notes

Small volumes of anti-MLH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MLH1 purchase

MBS850685 is an antibody targeting MLH1. Also known as MLH1 Antibody. Reactive in Human, Monkey. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MLH1. It may not necessarily be applicable to this product.

NCBI GI #
4505195
NCBI GeneID
4296
NCBI Accession #
NP_002410.1
UniProt Primary Accession #
Q16584
UniProt Secondary Accession #
Q53H00; Q59F06; Q6P2G4; B4DS76
UniProt Related Accession #
Q16584
GenBank Nucleotide #
NM_002419.3
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 11
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 11
NCBI Official Symbol
MAP3K11
NCBI Official Synonym Symbols
MLK3; PTK1; SPRK; MLK-3; MEKK11
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 11
UniProt Synonym Protein Names
Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase
Protein Family
DNA mismatch repair protein
UniProt Gene Name
MAP3K11
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 11

NCBI Summary for MLH1

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]
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UniProt Comments for MLH1

Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
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Research Articles on MLH1

1. In summary, this investigation identifies an EGFR-DOCK180-RAC1-MLK3-JNK signaling axis that drives glioblastoma cell migration and dissemination.IMPLICATIONS: On the basis of these findings, MLK3 emerges as a potential therapeutic target for the treatment of glioblastoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MLH1 antibody

 Reference Product  PubMed Publications
 MAP3K1 antibody  >1 publications with MLH1 and MAP3K1

Pathways associated with anti-MLH1 antibody

 Products by Pathway  Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 IFN-gamma Pathway antibodies  IFN-gamma Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Non-alcoholic Fatty Liver Disease (NAFLD) Pathway antibodies  Non-alcoholic Fatty Liver Disease (NAFLD) Pathway Diagram
 Non-alcoholic Fatty Liver Disease (NAFLD) Pathway antibodies  Non-alcoholic Fatty Liver Disease (NAFLD) Pathway Diagram
 RAC1 Signaling Pathway antibodies  RAC1 Signaling Pathway Diagram
 Reelin Signaling Pathway antibodies  Reelin Signaling Pathway Diagram

Diseases associated with anti-MLH1 antibody

 Disease Name  Pubmed Publications
 Hearing Loss Antibodies  >1 publications with MLH1 and Hearing Loss
 Nervous System Diseases Antibodies  >1 publications with MLH1 and Nervous System Diseases
 Immune System Diseases Antibodies  >1 publications with MLH1 and Immune System Diseases
 Breast Neoplasms Antibodies  >1 publications with MLH1 and Breast Neoplasms
 Inflammation Antibodies  >1 publications with MLH1 and Inflammation

Organs/Tissues associated with anti-MLH1 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >3 publications with MLH1 and Testis
 Blood Antibodies  >1 publications with MLH1 and Blood
 Adrenal Gland Antibodies  >1 publications with MLH1 and Adrenal Gland
 Uterus Antibodies  >1 publications with MLH1 and Uterus
 Prostate Antibodies  >1 publications with MLH1 and Prostate
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