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CHD3 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS850786

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CHD3, Antibody
Full Product Name
CHD3 Antibody
Product Gene Name
Product Synonym Names
ZFH; Mi-2a; Mi2-ALPHA; CHD3
Isotype
IgG1
Clone Number
2G4
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Molecular Weight
226kDa
OMIM
602120
3D Structure
ModBase 3D Structure for Q12873
Immunogen
Purified recombinant fragment of human CHD3 expressed in E. Coli.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CHD3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-CHD3 antibody
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000

Western Blot (WB) of anti-CHD3 antibody

Figure 1: Western blot analysis using CHD3 mouse mAb against Hela (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
Mouse Anti-CHD3 Antibody – Cat# MBS850786

Immunohistochemistry (IHC) of anti-CHD3 antibody

Figure 2: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CHD3 mouse mAb with DAB staining.
Mouse Anti-CHD3 Antibody, Immunohistochemistry (IHC) – Cat# MBS850786

Immunofluorescence (IF) of anti-CHD3 antibody

Figure 3: Immunofluorescence analysis of Hela cells using CHD3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Mouse Anti-CHD3 Antibody, Immunofluorescence (IF) – Cat# MBS850786

Other Notes

Small volumes of anti-CHD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHD3 purchase

MBS850786 is an antibody targeting CHD3. Also known as CHD3 Antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CHD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
chromodomain-helicase-DNA-binding protein 3 isoform 3
NCBI Official Synonym Full Names
chromodomain helicase DNA binding protein 3
NCBI Official Symbol
CHD3
NCBI Official Synonym Symbols
ZFH; Mi-2a; Mi2-ALPHA
UniProt Protein Name
Chromodomain-helicase-DNA-binding protein 3
UniProt Synonym Protein Names
ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH
UniProt Gene Name
CHD3
UniProt Synonym Gene Names
CHD-3; hZFH
NCBI Protein Information
chromodomain-helicase-DNA-binding protein 3

UniProt Comments for CHD3

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CHD3 antibody

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