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His-Tag Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS850940

MBS850940 is an antibody targeting His-Tag. Also known as His-Tag monoclonal antibody. Reactive in Human, Mouse, Rat. More details

$230 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
His-Tag, Monoclonal Antibody
Full Product Name
His-Tag monoclonal antibody
Clonality
Monoclonal
Clone Number
HIS.H8
Species Reactivity
Human, Mouse, Rat
Form/Format
1mg/ml in PBS (pH 7.4) with 0.05% sodium azide. Frozen liquid.
Specificity
His-tag monoclonal antibody recognizes His-tagged proteins overexpressed in cells, including both amino-or carboxy-termini of targeted proteins in transfected mammalian cells.
Preparation and Storage
Stable for 1 year at-20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4 degree C for 3 months.
Immunogen
6x His synthetic peptide
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-His-Tag antibody
Western Blot (WB), Immunoprecipitation (IP), Immunostaining
Application Notes for anti-His-Tag antibody
WB: 1:1000-4000
Immunoprecipitation:Immunostaining: 1:200-400.

Western Blot of anti-His-Tag antibody

His Western blot analysis of 293 cells transfected with His-tagged vector (2) and untransfected control (1).
Anti-His-Tag Monoclonal Antibody – Cat# MBS850940

Immunofluorescence of anti-His-Tag antibody

Immunofluorescence staining of His-tag fusion protein in 293 cells. A: Untr-ansfectsd control B; Transfected.
Anti-His-Tag Monoclonal Antibody, Immunofluorescence – Cat# MBS850940

Product References and Citations for anti-His-Tag antibody

Terpe K. et al. (2003) Appl Microbiol Biotechnol; 60(5):523-533. Xu L. et al. (2006) Cell Mol Immunol; 3(2):139-143. Els Conrath, K., et al. (2001) J. Biol. Chem. 276: 7346-7350.

Other Notes

Small volumes of anti-His-Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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