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NFIL3 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS851028

MBS851028 is an antibody targeting NFIL3. Also known as NFIL3 Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NFIL3, Antibody
Full Product Name
NFIL3 Antibody
Product Gene Name
Product Synonym Names
Nuclear factor interleukin-3-regulated protein; E4 promoter binding-protein 4; Interleukin-3 promoter transcriptional activator; Transcriptional activator NF-IL3A; Interleukin-3 binding-protein 1; E4BP4; IL3BP1
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
NFIL3 antibody detects endogenous levels of total NFIL3 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
51,472 Da
OMIM
605327
3D Structure
ModBase 3D Structure for Q16649
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human NFIL3.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-NFIL3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-NFIL3 antibody
WB: 1:500~1:1000
ELISA: 1:20000

Western Blot (WB) of anti-NFIL3 antibody

Western blot analysis of extracts from 293 cells and K562 cells, using NFIL3 antibody.
Anti-NFIL3 Antibody – Cat# MBS851028

Product References and Citations for anti-NFIL3 antibody

Cowell I.G., Mol. Cell. Biol. 12:3070-3077(1992). Zhang W., Mol. Cell. Biol. 15:6055-6063(1995). Humphray S.J., Nature 429:369-374(2004).

Other Notes

Small volumes of anti-NFIL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NFIL3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nuclear factor interleukin-3-regulated protein
NCBI Official Synonym Full Names
nuclear factor, interleukin 3 regulated
NCBI Official Symbol
NFIL3
NCBI Official Synonym Symbols
E4BP4; IL3BP1; NFIL3A; NF-IL3A
UniProt Protein Name
Nuclear factor interleukin-3-regulated protein
UniProt Synonym Protein Names
E4 promoter-binding protein 4; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; Transcriptional activator NF-IL3A
UniProt Gene Name
NFIL3
UniProt Synonym Gene Names
E4BP4; IL3BP1
NCBI Protein Information
nuclear factor interleukin-3-regulated protein

NCBI Summary for NFIL3

The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]
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UniProt Comments for NFIL3

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (). Represses transcriptional activity of PER1 (). Represses transcriptional activity of PER2 via the B-site on the promoter (). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (). Protects pro-B cells from programmed cell death ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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