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Keratin 14 (K14) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS851532

MBS851532 is an antibody targeting K14. Also known as Keratin 14 Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Keratin 14 (K14), Antibody
Full Product Name
Keratin 14 Antibody
Product Gene Name
Product Synonym Names
Keratin type I cytoskeletal 14; Cytokeratin-14; CK-14
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
Keratin 14 antibody detects endogenous levels of total Keratin 14 protein.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
51,561 Da
OMIM
125595
3D Structure
ModBase 3D Structure for P02533
Immunogen
The antiserum was produced against synthesized peptide derived from human Keratin 14.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-K14 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-K14 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000

Immunohistochemistry (IHC) of anti-K14 antibody

Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 14 antibody.
Anti-K14 Antibody – Cat# MBS851532

Western Blot (WB) of anti-K14 antibody

Western blot analysis of extracts from NIH/3T3 cells, using Keratin 14 antibody.
Anti-K14 Antibody, Western Blot (WB) – Cat# MBS851532

Immunofluorescence (IF) of anti-K14 antibody

Peptide-+ Immunofluorescence analysis of NIH/3T3 cells, using Keratin 14 antibody.
Anti-K14 Antibody, Immunofluorescence (IF) – Cat# MBS851532

Product References and Citations for anti-K14 antibody

Nam-On Ku, PNAS, Apr 2002; 99: 4373. E L Rugg, Genes & Dev., Nov 1994; 8: 2563-2573. Julia Reichelt, Mol. Biol. Cell, Jun 2001; 12: 1557. P. E. Bowden, Cancer Res., Nov 1992; 52: 5865-5871.

Other Notes

Small volumes of anti-K14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for K14. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type I cytoskeletal 14
NCBI Official Synonym Full Names
keratin 14
NCBI Official Symbol
KRT14
NCBI Official Synonym Symbols
K14; NFJ; CK14; EBS3; EBS4
UniProt Protein Name
Keratin, type I cytoskeletal 14
UniProt Synonym Protein Names
Cytokeratin-14; CK-14; Keratin-14; K14
UniProt Gene Name
KRT14
UniProt Synonym Gene Names
CK-14; K14
NCBI Protein Information
keratin, type I cytoskeletal 14

NCBI Summary for K14

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
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UniProt Comments for K14

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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