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RAS-GRF1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS851601

MBS851601 is an antibody targeting Ras-GRF1. Also known as Ras-GRF1 (Phospho-Ser916) Antibody. Reactive in Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ras-GRF1, Antibody
Full Product Name
Ras-GRF1 (Phospho-Ser916) Antibody
Product Gene Name
Product Synonym Names
CDC25; GNRP; GRF1; Guanine nucleotide releasing protein; P140 Ras-GRF; RASGRF1; Ras-specific nucleotide exchange factor CDC25
Species Reactivity
Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
Ras-GRF1 (Phospho-Ser916) antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
143,218 Da
OMIM
606600
3D Structure
ModBase 3D Structure for Q13972
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Ras-GRF1 around the phosphorylation site of serine 916 (R-M-SP-L-A).
All Sites
Mouse: Ser916
Rat: Ser898
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-Ras-GRF1 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-Ras-GRF1 antibody
IHC: 1:50-1:100
ELISA: 1:20000

Immunohistochemistry (IHC) of anti-Ras-GRF1 antibody

P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody.
Anti-RAS-GRF1 Antibody – Cat# MBS851601

Product References and Citations for anti-Ras-GRF1 antibody

Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278-13285. Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177-2189. Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379. Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816-14826.

Other Notes

Small volumes of anti-Ras-GRF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Ras-GRF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ras-specific guanine nucleotide-releasing factor 1 isoform 1
NCBI Official Synonym Full Names
Ras protein specific guanine nucleotide releasing factor 1
NCBI Official Symbol
RASGRF1
NCBI Official Synonym Symbols
GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
UniProt Protein Name
Ras-specific guanine nucleotide-releasing factor 1
UniProt Synonym Protein Names
Guanine nucleotide-releasing protein; GNRP; Ras-specific nucleotide exchange factor CDC25
UniProt Gene Name
RASGRF1
UniProt Synonym Gene Names
CDC25; GNRP; GRF1; Ras-GRF1; GNRP
NCBI Protein Information
ras-specific guanine nucleotide-releasing factor 1

NCBI Summary for Ras-GRF1

The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]
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UniProt Comments for Ras-GRF1

Promotes the exchange of Ras-bound GDP by GTP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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