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Caspase 10 (CASP-10) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS851625

MBS851625 is an antibody targeting CASP-10. Also known as Caspase 10 Antibody. Reactive in Human. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Caspase 10 (CASP-10), Antibody
Full Product Name
Caspase 10 Antibody
Product Gene Name
Product Synonym Names
ALPS2; CASP10B; FLICE2; Caspase 10 isoform a preproprotein; Caspase 10 isoform b preproprotein; Caspase 10 isoform c preproprotein; Caspase 10 isoform d preproprotein; FADD-LIKE ICE 2; FADD-like ICE2; Fas-associated death domain protein; ICE-like apoptotic protease 4; MCH4 CASPASE 10; ISOFORM B; apoptotic protease MCH-4; caspase 10; apoptosis-related cysteine protease; interleukin-1B-converting enzyme 2
Species Reactivity
Human
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
Caspase 10 antibody detects endogenous levels of total Caspase 10 protein.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
28,364 Da
OMIM
601762
3D Structure
ModBase 3D Structure for Q92851
Immunogen
The antiserum was produced against synthesized peptide derived from human Caspase 10.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CASP-10 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-CASP-10 antibody
WB: 1:300~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000

Immunohistochemistry (IHC) of anti-CASP-10 antibody

Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 10 antibody.
Anti-CASP-10 Antibody – Cat# MBS851625

Western Blot (WB) of anti-CASP-10 antibody

Western blot analysis of extracts from HeLa cells, using Caspase 10 antibody.
Anti-CASP-10 Antibody, Western Blot (WB) – Cat# MBS851625

Immunofluorescence (IF) of anti-CASP-10 antibody

Peptide-+ Immunofluorescence analysis of HeLa cells, using Caspase 10 antibody.
Anti-CASP-10 Antibody, Immunofluorescence (IF) – Cat# MBS851625

Product References and Citations for anti-CASP-10 antibody

Frank C. Kischkel, J. Biol. Chem., Nov 2001; 276: 46639-46646. Ingo H. Engels, Mol. Cell. Biol., Apr 2005; 25: 2808-2818. Daniela Sciaky, J. Immunol., Apr 2000; 164: 3806. Jin Wang, PNAS, Nov 2001; 98: 13884.

Other Notes

Small volumes of anti-CASP-10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CASP-10. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
caspase-10 isoform 6 preproprotein
NCBI Official Synonym Full Names
caspase 10
NCBI Official Symbol
CASP10
NCBI Official Synonym Symbols
MCH4; ALPS2; FLICE2
UniProt Protein Name
Caspase-10
UniProt Synonym Protein Names
Apoptotic protease Mch-4; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2
Protein Family
UniProt Gene Name
CASP10
UniProt Synonym Gene Names
MCH4; CASP-10; FLICE2
NCBI Protein Information
caspase-10

NCBI Summary for CASP-10

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]
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UniProt Comments for CASP-10

Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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