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MADD Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS851663

MBS851663 is an antibody targeting MADD. Also known as MADD Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MADD, Antibody
Full Product Name
MADD Antibody
Product Gene Name
Product Synonym Names
MAP kinase-activating death domain protein; Differentially expressed in normal and neoplastic cells; Insulinoma glucagonoma clone 20; Rab3 GDP/GTP exchange factor; MADD; DENN; IG20; KIAA0358
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
MADD antibody detects endogenous levels of total MADD protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
171,006 Da
OMIM
603584
3D Structure
ModBase 3D Structure for Q8WXG6
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human MADD.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MADD antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-MADD antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000

Testing Data of anti-MADD antibody

Peptide-+
Anti-MADD Antibody – Cat# MBS851663

Immunohistochemistry (IHC) of anti-MADD antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using MADD antibody. Western blot analysis of extracts from COLO cells, HeLa cells and Jurkat cells, using MADD antibody.
Anti-MADD Antibody, Immunohistochemistry (IHC) – Cat# MBS851663

Immunofluorescence (IF) of anti-MADD antibody

Peptide-+ Immunofluorescence analysis of A549 cells, using MADD antibody.
Anti-MADD Antibody, Immunofluorescence (IF) – Cat# MBS851663

Product References and Citations for anti-MADD antibody

Chow V.T.K., DNA Seq. 6:263-273(1996). Schievella A.R., J. Biol. Chem. 272:12069-12075(1997). Chow V.T.K., Genome 41:543-552(1998).

Other Notes

Small volumes of anti-MADD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MADD. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
MAP kinase-activating death domain protein isoform i
NCBI Official Synonym Full Names
MAP kinase activating death domain
NCBI Official Symbol
MADD
NCBI Official Synonym Symbols
DENN; IG20; RAB3GEP
UniProt Protein Name
MAP kinase-activating death domain protein
UniProt Synonym Protein Names
Differentially expressed in normal and neoplastic cells; Insulinoma glucagonoma clone 20; Rab3 GDP/GTP exchange factor
UniProt Gene Name
MADD
NCBI Protein Information
MAP kinase-activating death domain protein

NCBI Summary for MADD

Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for MADD

Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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