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Osteopontin (OPN) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS851678

MBS851678 is an antibody targeting OPN. Also known as Osteopontin Antibody. Host: Mouse. More details

$455 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Osteopontin (OPN), Monoclonal Antibody
Full Product Name
Osteopontin Antibody
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b
Host
Mouse
Form/Format
Each vial contains 100 ug IgG in 100 ul of citrate-tris-HCl buffer pH7.0 with 0.02% Proclin 300.
Specificity
Anti-Osteopontin recognizes recombinant protein Osteopontin.
Preparation and Storage
The antibody may be stored at-20°C for one year in its original formulation. Additionally, antibody may be stored at 2°C to 8°C for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.
OMIM
166490
3D Structure
ModBase 3D Structure for P10451
Host Note
Purified mouse monoclonal antibody generated using E Coli expressed recombinant protein Osteopontin as immunogen.
Antigen
Recombinant protein corresponding to full length Osteopontin.

Product Categories/Family for anti-OPN antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-OPN antibody
ELISA (EIA), Western Blot (WB-Ce)

Western Blot of anti-OPN antibody

WB (1:1000) analysis of Osteopontin expression in Jurkat whole cell lysate with Anti-Osteopontin (MBS851678).
Mouse Anti-OPN Monoclonal Antibody – Cat# MBS851678

Other Notes

Small volumes of anti-OPN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OPN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
osteopontin isoform OPN-a
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
UniProt Protein Name
Osteopontin
UniProt Synonym Protein Names
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
Protein Family
UniProt Gene Name
SPP1
UniProt Synonym Gene Names
BNSP; OPN; SPP-1
NCBI Protein Information
osteopontin

NCBI Summary for OPN

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for OPN

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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