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SF1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS852116

MBS852116 is an antibody targeting SF1. Also known as SF1 (Phospho-Ser82) Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SF1, Antibody
Full Product Name
SF1 (Phospho-Ser82) Antibody
Product Gene Name
Product Synonym Names
BBP; CW17; Mammalian branch point binding protein mBBP; SF01; Splicing factor 1; Splicing factor SF1; Transcription factor ZFM1; ZFM1; ZFP162; ZNF162; Zinc finger gene in MEN1 locus; Zinc finger protein 162; Mzfm
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
SF1 (Phospho-Ser82) antibody detects endogenous levels of SF1 only when phosphorylated at serine 82.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
65,371 Da
OMIM
601516
3D Structure
ModBase 3D Structure for Q15637
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human SF1 around the phosphorylation site of serine 82 (S-P-SP-P-E).
All Sites
Human: Ser82
Mouse: Ser82
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-SF1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-SF1 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000

Testing Data of anti-SF1 antibody

P-peptide-+
Anti-SF1 Antibody – Cat# MBS852116

Immunohistochemistry (IHC) of anti-SF1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using SF1 (Phospho-Ser82) antibody. Western blot analysis of extracts from HUVEC cells, treated with anisomycin (25ug/ml, 30mins), using SF1 (Phospho-Ser82) antibody.
Anti-SF1 Antibody, Immunohistochemistry (IHC) – Cat# MBS852116

Immunofluorescence (IF) of anti-SF1 antibody

P-peptide-+ Immunofluorescence analysis of A549 cells, using SF1 (Phospho-Ser82) antibody.
Anti-SF1 Antibody, Immunofluorescence (IF) – Cat# MBS852116

Product References and Citations for anti-SF1 antibody

Susan Y. Park, Development, May 2005; 132: 2415-2423. Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986. Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839. Philipp Gut, Development, Oct 2005; 132: 4611-4619.

Other Notes

Small volumes of anti-SF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
splicing factor 1 isoform 6
NCBI Official Synonym Full Names
splicing factor 1
NCBI Official Symbol
SF1
NCBI Official Synonym Symbols
BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25
UniProt Protein Name
Splicing factor 1
UniProt Synonym Protein Names
Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162
Protein Family
UniProt Gene Name
SF1
UniProt Synonym Gene Names
ZFM1; ZNF162; BBP; mBBP
NCBI Protein Information
splicing factor 1

NCBI Summary for SF1

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]
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UniProt Comments for SF1

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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