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PPHLN Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS852432

MBS852432 is an antibody targeting PPHLN. Also known as PPHLN Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PPHLN, Antibody
Full Product Name
PPHLN Antibody
Product Gene Name
Product Synonym Names
Periphilin-1; Gastric cancer antigen Ga50; PPHLN1; HSPC206; HSPC232
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
PPHLN antibody detects endogenous levels of total PPHLN protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
40,319 Da
OMIM
608150
3D Structure
ModBase 3D Structure for Q8NEY8
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human PPHLN.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PPHLN antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-PPHLN antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:5000

Testing Data of anti-PPHLN antibody

Peptide-+
Anti-PPHLN Antibody – Cat# MBS852432

Immunohistochemistry (IHC) of anti-PPHLN antibody

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using PPHLN antibody. Western blot analysis of extracts from COLO cells and 293 cells, using PPHLN antibody.
Anti-PPHLN Antibody, Immunohistochemistry (IHC) – Cat# MBS852432

Product References and Citations for anti-PPHLN antibody

Line A., Br. J. Cancer 86:1824-1830(2002). Kazerounian S., J. Biol. Chem. 278:36707-36717(2003). Zhang Q.-H., Genome Res. 10:1546-1560(2000).

Other Notes

Small volumes of anti-PPHLN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPHLN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
periphilin-1 isoform 6
NCBI Official Synonym Full Names
periphilin 1
NCBI Official Symbol
PPHLN1
NCBI Official Synonym Symbols
CR; HSPC206; HSPC232
UniProt Protein Name
Periphilin-1
UniProt Synonym Protein Names
CDC7 expression repressor
UniProt Gene Name
PPHLN1
UniProt Synonym Gene Names
CR
NCBI Protein Information
periphilin-1

NCBI Summary for PPHLN

The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
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UniProt Comments for PPHLN

Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin (PubMed:26022416). Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex (PubMed:15474462, PubMed:17963697). May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation (Probable).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPHLN antibody

Organs/Tissues associated with anti-PPHLN antibody

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