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ZAK Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS852588

MBS852588 is an antibody targeting ZAK. Also known as ZAK (Ab-165) Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ZAK, Antibody
Full Product Name
ZAK (Ab-165) Antibody
Product Gene Name
Product Synonym Names
AZK; EC 2.7.11.25; mixed lineage kinase-related kinase; MLTK; MRK-beta; sterile alpha motif and leucine zipper containing kinase AZK
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
ZAK (Ab-165) antibody detects endogenous levels of total ZAK protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
35,468 Da
OMIM
609479
3D Structure
ModBase 3D Structure for Q9NYL2
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human ZAK around the phosphorylation site of serine 165 (H-M-SP-L-V).
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ZAK antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ZAK antibody
WB: 1:500~1:1000
ELISA: 1:40000

Western Blot (WB) of anti-ZAK antibody

Western blot analysis of extracts from Jurkat cells, using ZAK (Ab-165) antibody.
Anti-ZAK Antibody – Cat# MBS852588

Product References and Citations for anti-ZAK antibody

Liu T.-C., Biochem. Biophys. Res. Commun. 274:811-816(2000). Bloem L.J., J. Mol. Cell. Cardiol. 33:1739-1750(2001). The MGC Project Team; Genome Res. 14:2121-2127(2004).

Other Notes

Small volumes of anti-ZAK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ZAK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 20 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 20
NCBI Official Symbol
MAP3K20
NCBI Official Synonym Symbols
pk; AZK; MLT; MRK; ZAK; CNM6; MLK7; MLTK; SFMMP; mlklak; MLTKbeta; MLTKalpha
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 20
UniProt Synonym Protein Names
Human cervical cancer suppressor gene 4 protein; HCCS-4; Leucine zipper- and sterile alpha motif-containing kinase; MLK-like mitogen-activated protein triple kinase; Mitogen-activated protein kinase kinase kinase MLT; Mixed lineage kinase-related kinase; MLK-related kinase; MRK; Sterile alpha motif- and leucine zipper-containing kinase AZK
UniProt Gene Name
MAP3K20
UniProt Synonym Gene Names
HCCS-4; MLK-related kinase; MRK
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 20

NCBI Summary for ZAK

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for ZAK

Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2 (PubMed:10924358, PubMed:11836244, PubMed:15342622, PubMed:21224381). Involved in limb development (PubMed:26755636).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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