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CHD4 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS852627

MBS852627 is an antibody targeting CHD4. Also known as CHD4 Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CHD4, Antibody
Full Product Name
CHD4 Antibody
Product Gene Name
Product Synonym Names
EC 3.6.1.-; ATP-dependent helicase CHD4; CHD-4; Mi-2 autoantigen 218 kDa protein; Mi2-beta
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
CHD4 antibody detects endogenous levels of total CHD4 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
220,848 Da
OMIM
603277
3D Structure
ModBase 3D Structure for Q14839
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human CHD4.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CHD4 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-CHD4 antibody
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:20000

Testing Data of anti-CHD4 antibody

Peptide-+
Anti-CHD4 Antibody – Cat# MBS852627

Immunohistochemistry (IHC) of anti-CHD4 antibody

Peptide-+ Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using CHD4 antibody. Immunofluorescence analysis of HepG2 cells, using CHD4 antibody.
Anti-CHD4 Antibody, Immunohistochemistry (IHC) – Cat# MBS852627

Product References and Citations for anti-CHD4 antibody

Seelig H.P., Arthritis Rheum. 38:1389-1399(1995). Schmidt D.R., Biochemistry 38:14711-14717(1999). Shimono Y., J. Biol. Chem. 278:51638-51645(2003).

Other Notes

Small volumes of anti-CHD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CHD4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
chromodomain-helicase-DNA-binding protein 4 isoform 1
NCBI Official Synonym Full Names
chromodomain helicase DNA binding protein 4
NCBI Official Symbol
CHD4
NCBI Official Synonym Symbols
CHD-4; Mi-2b; SIHIWES; Mi2-BETA
UniProt Protein Name
Chromodomain-helicase-DNA-binding protein 4
UniProt Synonym Protein Names
ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta
UniProt Gene Name
CHD4
UniProt Synonym Gene Names
CHD-4
NCBI Protein Information
chromodomain-helicase-DNA-binding protein 4

NCBI Summary for CHD4

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
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UniProt Comments for CHD4

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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