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Cytochrome P450 27a1 (CYP27A1) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS852683

MBS852683 is an antibody targeting CYP27A1. Also known as Cytochrome P450 27A1 Antibody. Reactive in Human. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytochrome P450 27A1 (CYP27A1), Antibody
Full Product Name
Cytochrome P450 27A1 Antibody
Product Gene Name
Product Synonym Names
25-hydroxylase; 5-beta-cholestane-3-alpha; 7-alpha; 12-alpha-triol 27-hydroxylase; CP27A; Cytochrome P-450C27/25; Cytochrome P450 27,mitochondrial; EC 1.14.13.15; Sterol 26-hydroxylase; Sterol 27-hydroxylase; Vitamin D
Species Reactivity
Human
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
Cytochrome P450 27A1 antibody detects endogenous levels of total Cytochrome P450 27A1 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
60,235 Da
OMIM
213700
3D Structure
ModBase 3D Structure for Q02318
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human Cytochrome P450 27A1.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CYP27A1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-CYP27A1 antibody
WB: 1:500-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-CYP27A1 antibody

Western blot analysis of extracts from HeLa cells, using Cytochrome P450 27A1 antibody.
Anti-CYP27A1 Antibody – Cat# MBS852683

Product References and Citations for anti-CYP27A1 antibody

Cali J.J., J. Biol. Chem. 266:7774-7778(1991). Guo Y.-D., Proc. Natl. Acad. Sci. U.S.A. 90:8668-8672(1993). Leitersdorf E., J. Clin. Invest. 91:2488-2496(1993).

Other Notes

Small volumes of anti-CYP27A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CYP27A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sterol 26-hydroxylase, mitochondrial
NCBI Official Synonym Full Names
cytochrome P450 family 27 subfamily A member 1
NCBI Official Symbol
CYP27A1
NCBI Official Synonym Symbols
CTX; CP27; CYP27
UniProt Protein Name
Sterol 26-hydroxylase, mitochondrial
UniProt Synonym Protein Names
5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase
Protein Family
UniProt Gene Name
CYP27A1
UniProt Synonym Gene Names
CYP27
NCBI Protein Information
sterol 26-hydroxylase, mitochondrial

NCBI Summary for CYP27A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008]
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UniProt Comments for CYP27A1

Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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