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MED12 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS852732

MBS852732 is an antibody targeting MED12. Also known as MED12 Antibody. Reactive in Human. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MED12, Antibody
Full Product Name
MED12 Antibody
Product Gene Name
Product Synonym Names
Thyroid hormone receptor-associated protein complex 230 kDa component; Trap230; Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; OPA-containing protein; Trinucleotide repeat-containing gene 11 protein
Species Reactivity
Human
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
MED12 antibody detects endogenous levels of total MED12 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
242,953 Da
OMIM
300188
3D Structure
ModBase 3D Structure for Q93074
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human MED12.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MED12 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-MED12 antibody
IHC: 1:50-1:100
ELISA: 1:5000

Immunohistochemistry (IHC) of anti-MED12 antibody

Peptide-+ Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using MED12 antibody.
Anti-MED12 Antibody – Cat# MBS852732

Product References and Citations for anti-MED12 antibody

Ito M., Mol. Cell 3:361-370(1999). Nagase T., DNA Res. 3:17-24(1996). Philibert R.A., Mol. Psychiatry 3:303-309(1998).

Other Notes

Small volumes of anti-MED12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MED12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 12
NCBI Official Synonym Full Names
mediator complex subunit 12
NCBI Official Symbol
MED12
NCBI Official Synonym Symbols
OKS; FGS1; HOPA; OPA1; OHDOX; ARC240; CAGH45; MED12S; TNRC11; TRAP230
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 12
UniProt Synonym Protein Names
Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; Mediator complex subunit 12; OPA-containing protein; Thyroid hormone receptor-associated protein complex 230 kDa component; Trap230; Trinucleotide repeat-containing gene 11 protein
UniProt Gene Name
MED12
UniProt Synonym Gene Names
ARC240; CAGH45; HOPA; KIAA0192; TNRC11; TRAP230; ARC240; Trap230
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 12

NCBI Summary for MED12

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]
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UniProt Comments for MED12

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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