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ITGA5 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS853047

MBS853047 is an antibody targeting ITGA5. Also known as ITGA5 (light chain, Cleaved-Glu895) Antibody. Reactive in Human. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ITGA5, Antibody
Full Product Name
ITGA5 (light chain, Cleaved-Glu895) Antibody
Product Gene Name
Product Synonym Names
Integrin alpha-5 [Precursor]; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD49e antigen; Integrin alpha-5 heavy chain; Integrin alpha-5 light chain; ITGA5; FNRA
Species Reactivity
Human
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
ITGA5 (light chain, Cleaved-Glu895) antibody detects endogenous levels of fragment of activated ITGA5 resulting from cleavage adjacent to Glu895.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
114,536 Da
OMIM
135620
3D Structure
ModBase 3D Structure for P08648
Immunogen
The antiserum was produced against synthesized peptide derived from human ITGA5.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ITGA5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ITGA5 antibody
WB: 1:500~1:1000
ELISA: 1:20000

Western Blot (WB) of anti-ITGA5 antibody

Western blot analysis of extracts from 293 cells treated with etoposide (25uM, 1hour) and A549 cells treated with etoposide (25uM, 1hour), using ITGA5 (light chain, Cleaved-Glu895) antibody.
Anti-ITGA5 Antibody – Cat# MBS853047

Product References and Citations for anti-ITGA5 antibody

Argraves W.S., J. Cell Biol. 105:1183-1190(1987). The MGC Project Team; Genome Res. 14:2121-2127(2004). Birkenmeier T.M., J. Biol. Chem. 266:20544-20549(1991).

Other Notes

Small volumes of anti-ITGA5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ITGA5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
integrin alpha-5
NCBI Official Synonym Full Names
integrin subunit alpha 5
NCBI Official Symbol
ITGA5
NCBI Official Synonym Symbols
FNRA; CD49e; VLA-5; VLA5A
UniProt Protein Name
Integrin alpha-5
UniProt Synonym Protein Names
CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD_antigen: CD49e
Protein Family
UniProt Gene Name
ITGA5
UniProt Synonym Gene Names
FNRA
NCBI Protein Information
integrin alpha-5

NCBI Summary for ITGA5

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015]
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UniProt Comments for ITGA5

Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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