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AOS1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS853062

MBS853062 is an antibody targeting AOS1. Also known as AOS1 Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AOS1, Antibody
Full Product Name
AOS1 Antibody
Product Gene Name
Product Synonym Names
AOS1; HSPC140; SAE1; SUA1; SUMO-1 activating enzyme E1 N subunit; ULE1A; UBLE1A
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
AOS1 antibody detects endogenous levels of total AOS1 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
33,063 Da
OMIM
613294
3D Structure
ModBase 3D Structure for Q9UBE0
Immunogen
The antiserum was produced against synthesized peptide derived from Internal of human AOS1.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-AOS1 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-AOS1 antibody
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:20000

Testing Data of anti-AOS1 antibody

Anti-AOS1 Antibody – Cat# MBS853062

Western Blot (WB) of anti-AOS1 antibody

Peptide-+ Western blot analysis of extracts from 293 cells, using AOS1 antibody. Immunofluorescence analysis of HuvEc cells, using AOS1 antibody.
Anti-AOS1 Antibody, Western Blot (WB) – Cat# MBS853062

Product References and Citations for anti-AOS1 antibody

Yoshiaki Azuma, FASEB J, Jun 2001; 10.1096. Walter Makous, Invest. Ophthalmol. Vis. Sci., Sep 2006; 47: 4160-4167. Hsin-Pei Shih, J. Cell Sci., Mar 2002; 115: 1259. Thomas Buschmann, J. Biol. Chem., Oct 2001; 276: 40389-40395.

Other Notes

Small volumes of anti-AOS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AOS1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
SUMO-activating enzyme subunit 1 isoform b
NCBI Official Synonym Full Names
SUMO1 activating enzyme subunit 1
NCBI Official Symbol
SAE1
NCBI Official Synonym Symbols
AOS1; SUA1; UBLE1A; HSPC140
UniProt Protein Name
SUMO-activating enzyme subunit 1
UniProt Synonym Protein Names
Ubiquitin-like 1-activating enzyme E1A
UniProt Gene Name
SAE1
UniProt Synonym Gene Names
AOS1; SUA1; UBLE1A
NCBI Protein Information
SUMO-activating enzyme subunit 1

NCBI Summary for AOS1

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
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UniProt Comments for AOS1

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AOS1 antibody

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