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PLD1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS853109

MBS853109 is an antibody targeting PLD1. Also known as PLD1 (Phospho-Thr147) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PLD1, Antibody
Full Product Name
PLD1 (Phospho-Thr147) Antibody
Product Gene Name
Product Synonym Names
Phospholipase D1; PLD 1; hPLD1; EC=3.1.4.4; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
PLD1 (Phospho-Thr147) antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
112,184 Da
OMIM
212093
3D Structure
ModBase 3D Structure for Q13393
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human PLD1 around the phosphorylation site of threonine 147.
All Sites
Human: Thr147
Mouse: Thr147
Rat: Thr147
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PLD1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-PLD1 antibody
WB: 1:500~1:1000
ELISA: 1:40000

Western Blot (WB) of anti-PLD1 antibody

Western blot analysis of extracts from HepG2 cells, using PLD1 (Phospho-Thr147) antibody.
Anti-PLD1 Antibody – Cat# MBS853109

Product References and Citations for anti-PLD1 antibody

Hammond S.M., J. Biol. Chem. 272:3860-3868(1997) [PubMed: 9013646]. Steed P.M., FASEB J. 12:1309-1317(1998) [PubMed: 9761774]. Lopez I., J. Biol. Chem. 273:12846-12852(1998) [PubMed: 9582313].

Other Notes

Small volumes of anti-PLD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PLD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phospholipase D1 isoform a
NCBI Official Synonym Full Names
phospholipase D1
NCBI Official Symbol
PLD1
NCBI Official Synonym Symbols
CVDD
UniProt Protein Name
Phospholipase D1
UniProt Synonym Protein Names
Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
UniProt Gene Name
PLD1
UniProt Synonym Gene Names
PLD 1; hPLD1
NCBI Protein Information
phospholipase D1

NCBI Summary for PLD1

This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011]
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UniProt Comments for PLD1

Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic ().

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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