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ATG4A Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS853510

MBS853510 is an antibody targeting ATG4A. Also known as ATG4A Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATG4A, Antibody
Full Product Name
ATG4A Antibody
Product Gene Name
Product Synonym Names
Cysteine protease ATG4A; EC 3.4.22.-; Autophagy-related protein 4 homolog A; hAPG4A; Autophagin-2; Autophagy-related cysteine endopeptidase 2; AUT-like 2 cysteine endopeptidase
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
ATG4A antibody detects endogenous levels of total ATG4A protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
42,453 Da
OMIM
300663
3D Structure
ModBase 3D Structure for Q8WYN0
Immunogen
The antiserum was produced against synthesized peptide derived from C-terminal of human ATG4A.

Product Categories/Family for anti-ATG4A antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ATG4A antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-ATG4A antibody
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:20000

Testing Data of anti-ATG4A antibody

Peptide-+
Anti-ATG4A Antibody – Cat# MBS853510

Immunohistochemistry (IHC) of anti-ATG4A antibody

Peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ATG4A antibody. Immunofluorescence analysis of A549 cells, using ATG4A antibody.
Anti-ATG4A Antibody, Immunohistochemistry (IHC) – Cat# MBS853510

Product References and Citations for anti-ATG4A antibody

Marino G., J. Biol. Chem. 278:3671-3678(2003). Kabeya Y., J. Cell Sci. 117:2805-2812(2004). Ross M.T., Nature 434:325-337(2005).

Other Notes

Small volumes of anti-ATG4A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG4A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cysteine protease ATG4A isoform c
NCBI Official Synonym Full Names
autophagy related 4A cysteine peptidase
NCBI Official Symbol
ATG4A
NCBI Official Synonym Symbols
APG4A; AUTL2
UniProt Protein Name
Cysteine protease ATG4A
UniProt Synonym Protein Names
AUT-like 2 cysteine endopeptidase; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; hAPG4A
Protein Family
UniProt Gene Name
ATG4A
UniProt Synonym Gene Names
APG4A; AUTL2; hAPG4A
NCBI Protein Information
cysteine protease ATG4A

NCBI Summary for ATG4A

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq, Mar 2016]
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UniProt Comments for ATG4A

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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