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KLHL3 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS853527

MBS853527 is an antibody targeting KLHL3. Also known as KLHL3 Antibody. Reactive in Human. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KLHL3, Antibody
Full Product Name
KLHL3 Antibody
Product Gene Name
Product Synonym Names
Kelch-like protein 3; KIAA1129
Species Reactivity
Human
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
KLHL3 antibody detects endogenous levels of total KLHL3 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
55,927 Da
OMIM
605775
3D Structure
ModBase 3D Structure for Q9UH77
Immunogen
The antiserum was produced against synthesized peptide derived from N-terminal of human KLHL3.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-KLHL3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-KLHL3 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000

Testing Data of anti-KLHL3 antibody

Peptide-+
Anti-KLHL3 Antibody – Cat# MBS853527

Immunohistochemistry (IHC) of anti-KLHL3 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KLHL3 antibody. Western blot analysis of extracts from RAW264.7 cells and 3T3 cells, using KLHL3 antibody.
Anti-KLHL3 Antibody, Immunohistochemistry (IHC) – Cat# MBS853527

Immunofluorescence (IF) of anti-KLHL3 antibody

Peptide-+ Immunofluorescence analysis of A549 cells, using KLHL3 antibody.
Anti-KLHL3 Antibody, Immunofluorescence (IF) – Cat# MBS853527

Product References and Citations for anti-KLHL3 antibody

Lai F., Genomics 66:65-75(2000). Hirosawa M., DNA Res. 6:329-336(1999). Ota T., Nat. Genet. 36:40-45(2004).

Other Notes

Small volumes of anti-KLHL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KLHL3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
kelch-like protein 3 isoform 2
NCBI Official Synonym Full Names
kelch like family member 3
NCBI Official Symbol
KLHL3
NCBI Official Synonym Symbols
PHA2D
UniProt Protein Name
Kelch-like protein 3
Protein Family
UniProt Gene Name
KLHL3
NCBI Protein Information
kelch-like protein 3

NCBI Summary for KLHL3

This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
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UniProt Comments for KLHL3

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron (PubMed:14528312, PubMed:22406640, PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031). The BCR(KLHL3) complex acts by mediating ubiquitination of WNK4, an inhibitor of potassium channel KCNJ1, leading to WNK4 degradation (PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031). The BCR(KLHL3) complex also mediates ubiquitination and degradation of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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