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ILK Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS853648

MBS853648 is an antibody targeting ILK. Also known as ILK (Phospho-Ser246) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ILK, Antibody
Full Product Name
ILK (Phospho-Ser246) Antibody
Product Gene Name
Product Synonym Names
59 kDa serine/threonine protein kinase; EC 2.7.11.1; ILK-1; ILK1; Integrin-linked protein kinase; Integrin-linked protein kinase 1; kinase ILK; p59ILK
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
ILK (Phospho-Ser246) antibody detects endogenous levels of ILK only when phosphorylated at serine 246.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
36,467 Da
OMIM
602366
3D Structure
ModBase 3D Structure for Q13418
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ILK around the phosphorylation site of serine 246 (I-F-SP-H-P).
All Sites
Human: Ser246
Mouse: Ser246
Rat: Ser246
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ILK antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-ILK antibody
IHC: 1:50-1:100
ELISA: 1:1000

Immunohistochemistry (IHC) of anti-ILK antibody

P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using ILK (Phospho-Ser246) antibody.
Anti-ILK Antibody – Cat# MBS853648

Product References and Citations for anti-ILK antibody

Hannigan G.E., Nature 379:91-96(1996). Janji B., Oncogene 19:3069-3077(2000). Tadic B., Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.

Other Notes

Small volumes of anti-ILK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ILK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
integrin-linked protein kinase isoform 1
NCBI Official Synonym Full Names
integrin linked kinase
NCBI Official Symbol
ILK
NCBI Official Synonym Symbols
P59; ILK-1; ILK-2; p59ILK; HEL-S-28
UniProt Protein Name
Integrin-linked protein kinase
UniProt Synonym Protein Names
59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK
UniProt Gene Name
ILK
UniProt Synonym Gene Names
ILK1; ILK2
NCBI Protein Information
integrin-linked protein kinase

NCBI Summary for ILK

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
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UniProt Comments for ILK

Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715). May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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