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MEKKK 4 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS853687

MBS853687 is an antibody targeting MEKKK 4. Also known as MEKKK 4 Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MEKKK 4, Antibody
Full Product Name
MEKKK 4 Antibody
Product Gene Name
Product Synonym Names
EC 2.7.11.1; HPK/GCK-like kinase HGK; M4K4; MAP4K4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck interacting kinase; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; mitogen-activated protein kinase kinase kinase kinase 4
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
MEKKK 4 antibody detects endogenous levels of total MEKKK 4 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
145,005 Da
OMIM
604666
3D Structure
ModBase 3D Structure for O95819
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human MEKKK 4.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MEKKK 4 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-MEKKK 4 antibody
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:5000

Testing Data of anti-MEKKK 4 antibody

Anti-MEKKK 4 Antibody – Cat# MBS853687

Western Blot (WB) of anti-MEKKK 4 antibody

Peptide-+ Western blot analysis of extracts from COLO cells and HUVEC cells, using MEKKK 4 antibody. Immunofluorescence analysis of A549 cells, using MEKKK 4 antibody.
Anti-MEKKK 4 Antibody, Western Blot (WB) – Cat# MBS853687

Product References and Citations for anti-MEKKK 4 antibody

Yao Z., J. Biol. Chem. 274:2118-2125(1999). Wright J.H., Mol. Cell. Biol. 23:2068-2082(2003). Ishikawa K., DNA Res. 5:169-176(1998).

Other Notes

Small volumes of anti-MEKKK 4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MEKKK 4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase kinase 4
NCBI Official Symbol
MAP4K4
NCBI Official Synonym Symbols
HGK; NIK; MEKKK4; FLH21957; HEL-S-31
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase kinase 4
UniProt Synonym Protein Names
HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck-interacting kinase
UniProt Gene Name
MAP4K4
UniProt Synonym Gene Names
HGK; KIAA0687; NIK; MEK kinase kinase 4; MEKKK 4
NCBI Protein Information
mitogen-activated protein kinase kinase kinase kinase 4

NCBI Summary for MEKKK 4

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
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UniProt Comments for MEKKK 4

Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MEKKK 4 antibody

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