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Patched (PTCH) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS854257

MBS854257 is an antibody targeting PTCH. Also known as Patched Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Patched (PTCH), Antibody
Full Product Name
Patched Antibody
Product Gene Name
Product Synonym Names
DROSOPHILA HOMOLOG OF 1; patched homolog (Drosophila)
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
Patched antibody detects endogenous levels of total Patched protein.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
144,356 Da
OMIM
109400
3D Structure
ModBase 3D Structure for Q13635
Immunogen
The antiserum was produced against synthesized peptide derived from human Patched.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PTCH antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-PTCH antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000

Immunohistochemistry (IHC) of anti-PTCH antibody

Peptide-+ Immunohistochemical analysis of paraffin-embedded human brain tissue using Patched antibody.
Anti-PTCH Antibody – Cat# MBS854257

Western Blot (WB) of anti-PTCH antibody

Western blot analysis of extracts from mouse muscle cells, using Patched antibody.
Anti-PTCH Antibody, Western Blot (WB) – Cat# MBS854257

Product References and Citations for anti-PTCH antibody

Trudy G. Oliver, Development, May 2005; 132: 2425-2439. Florence Brellier, Cancer Res., Apr 2004; 64: 2699-2704. Heidi Hahn, J. Biol. Chem., May 1996; 271: 12125. RL Johnson, Development, Dec 1995; 121: 4161.

Other Notes

Small volumes of anti-PTCH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PTCH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein patched homolog 1 isoform L
NCBI Official Synonym Full Names
patched 1
NCBI Official Symbol
PTCH1
NCBI Official Synonym Symbols
PTC; BCNS; PTC1; PTCH; NBCCS
UniProt Protein Name
Protein patched homolog 1
UniProt Gene Name
PTCH1
UniProt Synonym Gene Names
PTCH; PTC; PTC1
NCBI Protein Information
protein patched homolog 1

NCBI Summary for PTCH

This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017]
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UniProt Comments for PTCH

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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