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DNA Polymerase Beta (POLB) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS854267

MBS854267 is an antibody targeting POLB. Also known as DNA Polymerase beta Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNA Polymerase beta (POLB), Antibody
Full Product Name
DNA Polymerase beta Antibody
Product Gene Name
Product Synonym Names
DNA polymerase beta; EC 2.7.7.7; EC 4.2.99
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
DNA Polymerase beta antibody detects endogenous levels of total DNA Polymerase beta protein.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
38,178 Da
OMIM
174760
3D Structure
ModBase 3D Structure for P06746
Immunogen
The antiserum was produced against synthesized peptide derived from human DNA Polymerase beta.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-POLB antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-POLB antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000

Immunohistochemistry (IHC) of anti-POLB antibody

Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DNA Polymerase beta antibody.
Anti-POLB Antibody – Cat# MBS854267

Western Blot (WB) of anti-POLB antibody

Western blot analysis of extracts from NIH/3T3 cells, using DNA Polymerase beta antibody.
Anti-POLB Antibody, Western Blot (WB) – Cat# MBS854267

Product References and Citations for anti-POLB antibody

Hiroyuki Matsumoto, Plant Cell Physiol., Nov 1981; 22: 1317-1324. L.K. Karagyozov, Nucleic Acids Res., Jun 1978; 5: 1907-1917. F Costanzo, J. Virol., Mar 1977; 21: 996-1001. AJ Faber, Eur. J. Biochem., Nov 1981; 120: 357.

Other Notes

Small volumes of anti-POLB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POLB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA polymerase beta
NCBI Official Synonym Full Names
DNA polymerase beta
NCBI Official Symbol
POLB
UniProt Protein Name
DNA polymerase beta
Protein Family
UniProt Gene Name
POLB
NCBI Protein Information
DNA polymerase beta

NCBI Summary for POLB

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]
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UniProt Comments for POLB

Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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