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Cullin 2 (CUL-2) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS854401

MBS854401 is an antibody targeting CUL-2. Also known as Cullin 2 Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cullin 2 (CUL-2), Antibody
Full Product Name
Cullin 2 Antibody
Product Gene Name
Product Synonym Names
Cullin-2
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
Cullin 2 antibody detects endogenous levels of total Cullin 2 protein.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
89,492 Da
OMIM
603135
3D Structure
ModBase 3D Structure for Q13617
Immunogen
The antiserum was produced against synthesized peptide derived from human Cullin 2.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CUL-2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-CUL-2 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000

Immunohistochemistry (IHC) of anti-CUL-2 antibody

Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cullin 2 antibody.
Anti-CUL-2 Antibody – Cat# MBS854401

Western Blot (WB) of anti-CUL-2 antibody

Western blot analysis of extracts from LOVO cells, using Cullin 2 antibody.
Anti-CUL-2 Antibody, Western Blot (WB) – Cat# MBS854401

Immunofluorescence (IF) of anti-CUL-2 antibody

Peptide-+ Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 antibody.
Anti-CUL-2 Antibody, Immunofluorescence (IF) – Cat# MBS854401

Product References and Citations for anti-CUL-2 antibody

Seiji Shiraishi, J. Biol. Chem., Mar 2007; 282: 9017-9028. Isabelle Groulx, J. Biol. Chem., Mar 2000; 275: 8991. Dimitris Liakopoulos, PNAS, May 1999; 96: 5510. Kyungwon Huh, AACR Meeting Abstracts, Apr 2006; 2006: 1326.

Other Notes

Small volumes of anti-CUL-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CUL-2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cullin-2 isoform c
NCBI Official Synonym Full Names
cullin 2
NCBI Official Symbol
CUL2
UniProt Protein Name
Cullin-2
Protein Family
UniProt Gene Name
CUL2
UniProt Synonym Gene Names
CUL-2
NCBI Protein Information
cullin-2

UniProt Comments for CUL-2

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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